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  1. Laboratory Methods (Food)

BAM Media M147: Thiosulfate-Citrate-Bile Salts-Sucrose (TCBS) Agar

January 2001

Bacteriological Analytical Manual

Yeast extract 5 g Oxgall 5 g
Peptone 10 g NaCl 10 g
Sucrose 20 g Ferric citrate 1 g
Sodium thiosulfate 5H2O 10 g Bromthymol blue 0.04 g
Sodium citrate 2H2O 10 g Thymol blue 0.04 g
Sodium cholate 3 g Agar 15 g
Distilled water 1 liter

Prepare in flask at least 3 times larger than required volume of medium. Add ingredients to warm distilled water and heat to dissolve. Bring just to boil, and immediately remove from heat. DO NOT AUTOCLAVE! Cool to 50°C and pour into petri dishes. Dry the plates overnight or at 37-45°C before use.

Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.