Andriy Tkachenko PhD.

Leadership Role

Division of One Health Monitoring

Biography

Andriy Tkachenko received his bachelor’s degree in 1997 from the National Agricultural University, Ukraine, where he researched the behavior of radioactive strontium and cesium in the plant-soil system. In 2002, he received his master’s degree from the University of Manchester, United Kingdom jointly with the Central European University, Hungary, where he investigated the behavior and toxicity of metals in aquatic animals. In 2010, he earned his PhD from the University of Maryland Eastern Shore, where he characterized the susceptibility of aquatic animals to environmental stressors and diseases using chemical and biochemical approaches in combination with analytical equipment.

Dr. Tkachenko joined FDA in 2010 as an Oak Ridge Associated Universities (ORAU) fellow, working primarily on setting up analytical instrumentation for the newly established Veterinary Laboratory Investigation and Response Network (Vet-LIRN). Dr. Tkachenko performed chemical analysis of research and investigational samples. In 2025, Dr. Tkachenko became a Consumer Safety Officer (CSO) in Vet-LIRN.

Research

As a CSO, Dr. Tkachenko (i) coordinates communications between Vet-LIRN Collaborative Agreement Program (CAP) funded laboratories and the Vet-LIRN program office; (ii) provides technical assistance, advice, and guidance in development and validation of chemistry and microbiology methods by CAP laboratories according to FDA, International Organization for Standardization (ISO) and Association of Official Analytical Chemists (AOAC) Guidelines; (iii) reviews results and reports on progress of CAP laboratory method validation projects; (iv) designs, organizes, conducts, and evaluates method testing exercises called Blinded Method Tests (BMTs) for individual CAP laboratory projects, and (v) assists in designing, organizing, and conducting activities including evaluation of results under the Proficiency Test program for Vet-LIRN Network Laboratories in collaboration with the Moffett Campus, FDA.

Select Publications

B. Fritz, M. Kleinhenz, G. Magnin, J. Griffin, M. Weeder, A. Curtis, M. Martin, A. Nelson, K. Kleinhenz, B. Johnson, S. Fritz, S. Montgomery, A. Tkachenko, J. Coetzee. Tissue residue depletion of cannabinoids in cattle administered industrial hemp inflorescence. Scientific Reports. 2025.

S. Nemser, O. Ceric, J. Guag, S. Pauley, A. Jones, K. Proia, M. Miller, A. Tkachenko, David Rotstein, April Hodges, Renate Reimschuessel, Gregory H. Tyson. The Veterinary Laboratory Investigation and Response Network: 15 Years of Promoting Human and Animal Health by Collaborating with the Veterinary Diagnostic Laboratory Community. Journal of Food Protection. 2025.

A. Pepper, S. Kayastha, M. Miller, J. Guag, A. Tkachenko, M. Allender, K. Terio, L. Wang. Evaluation of RT-LAMP for SARS-CoV-2 Detection in Animal Feces. Viruses. 2025.

A. Tkachenko, Y. Chen, M. Petrey, S. Fritz, T. Walsh, D. Rotstein, M. Miller, B. Williams, M. Dark, M. Kmet, R. Reddy, S. Nemser. A Novel Proficiency Test to Assess the Diagnostic Investigation Process in Identifying an Unknown Toxicant. Toxicology Reports. 2025.

S. Kiener, E. Smith, N. Singh, S. Nemser, K. Hettwer, M. Miller, A. Tkachenko, S. Uhlig, R. Reddy. Determination of limit of detection and relative limit of detection of Salmonella in Raw Pet Food Matrices Using Salmonella Bacteriological Analytical Manual Methods. Journal of Microbiological Methods. 2025.

N. Singh, M. Miller, S. Nemser, A. Tkachenko, S. Uhlig, K. Frost, K. Hettwer, J. Ulaszek, M. Kmet, R. Reddy. Proficiency Test of SARS-CoV-2 Omicron Variant Detection in Diagnostics Samples by Veterinary Diagnostic Laboratories. Accreditation and Quality Assurance. 2025.

A. Leonardi-Cattolica, S. Kayastha, M. Miller, J. Guag, A. Tkachenko, J. Lowe, M. Allender, K. Terio, L. Wang. Evaluation of fecal sample pooling for real-time RT-PCR testing SARS-CoV-2 in animals. Viruses. 2024.

J. Langston, S. Stump, M. Filigenzi, A. Tkachenko, J. Guag, R. Poppenga, W. Rumbeiha. Extensive evaluation of a new LC-MS/MS method to quantify monofluoroacetate toxin in the kidney. Journal of Analytical Toxicology. 2024.

M. Miller, A. Tkachenko, J. Guag S. Alexander, B. Webb, B. Stenger. Comparative evaluation of assay performance for SARS-CoV-2 detection in animal oral samples, lung homogenates, and phosphate-buffered saline using the Applied Biosystems TaqPath COVID-19 Combo kit. Journal of Veterinary Diagnostic Investigation. 2024.

Y. Chen, S. Lopez, R, M. Reddy, J. Wan, A. Tkachenko, S. Nemser, L. Smith, R. Reimschuessel. Validation and Interlaboratory Comparison of an UPLC-MS/MS Method for Determination of Anticoagulant Rodenticides in Animal Livers. Journal of Veterinary Diagnostic Investigation. 2023.

K. Francis, A. Tkachenko, J. Johnson, L. Smith, R. Noonan, M. Filigenzi, C. Gaskill and M. Romano. Comprehensive Evaluation of HPLC-MS/MS Method for Quantitation of Seven Anticoagulant Rodenticides and Dicoumarol in Animal Serum. Journal of Analytical Toxicology. 2023.

X. Du, D. Schrunk, P. Imerman, J. Tahara, A. Tkachenko, J. Guag, R. Reimschuessel and W. Rumbeiha. Extensive Evaluation of a Method for Quantitative Measurement of Aflatoxins B1 and M1 in Animal Urine Using High-Performance Liquid Chromatography with Fluorescence Detection. Journal of AOAC International. 2023.

K. Deng, S. M. Nemser, K. Frost, L. Goodman, H. Ip, M. Killian, J. Ulaszek, S. Kiener, M. Kmet, S. Uhlig, K. Hettwer, B. Colson, K. Nichani, A. Schlierf, A. Tkachenko, M. Miller, R. Reddy, G. Tyson. Successful Detection of Delta and Omicron Variants of SARS-CoV-2 by Veterinary Diagnostic Laboratory Participants in an Interlaboratory Comparison Exercise. The Journal of Applied Laboratory Medicine. 2023

K. Deng, S. Uhlig, L. Goodman, H. Ip, M. Killian, Sarah M. Nemser, J. Ulaszek, S. Kiener, M. Kmet, K. Frost, K. Hettwer, B. Colson, K. Nichani, A. Schlierf, A. Tkachenko, M. Mlalazi-Oyinloye, A. Scott, R. Reddy, G. Tyson. Second round of the interlaboratory comparison (ILC) exercise 1 of SARS-CoV-2 molecular detection assays being used by 45 veterinary diagnostic laboratories in the US. Journal of Veterinary Diagnostic Investigation. 2022.

A. Tkachenko, K. Benson, M. Mostrom, J. Guag, R. Reimschuessel and B. Webb. Extensive evaluation via blinded testing of an UHPLC-MS/MS method for quantitation of ten ergot alkaloids in rye and wheat grains. Journal of AOAC International. 2021.

K. Deng, S. Uhlig, H. Ip, M. L. Killian, L. Goodman, S. Nemser, J. Ulaszek, S. Pickens, R. Newkirk, M. Kmet, K. Frost, K. Hettwer, B. Colson, K. Nichani, A. Schlierf, A. Tkachenko, R. Reddy and R. Reimschuessel. Interlaboratory comparison of SARS-CoV2 molecular detection assays in use by U.S. veterinary diagnostic laboratories. Journal of Veterinary Diagnostic Investigation. 2021.

S. Nemser, S. Lindemann, Y. Chen, S. Lopez, S. Pickens, J. Ulaszek, M. Kmet, C. Powers, S. Ensley, D. Schrunk, W. Rumbeiha, A. Tkachenko, J. Guag, O. Ceric, J. Jones, R. Reimschuessel, R. Reddy. A Review of Proficiency Exercises Offered by the Veterinary Laboratory Investigation and Response Network and Moffett Proficiency Testing Laboratory from 2012 to 2018. Accreditation and Quality Assurance. 2021.

D. Vudathala, M. Cummings, A. Tkachenko, J. Guag, R. Reimschuessel, L. Murphy. A Lateral Flow Method for Aflatoxin B1 in Dry Dog Food: An Inter-Laboratory Trial. Journal of AOAC International. 2021.

Contact Information: Andriy Tkachenko; +1 (240) 402-5420; +1 (301) 210-4685