BAM R57: Peroxidase Substrate for Membrane ELISA

 

January 2001

Solution A

4-Chloro-1-naphthol	60 mg
Methanol (cold)	20 ml

Dissolve 4-chloro-1-naphthol in cold methanol. Store at -20°C.

Solution B

Hydrogen peroxide, 30%	60 1
Tris-buffered saline (TBS)	100 ml

Add 30% H₂O₂ to TBS at room temperature.

Immediately before use, mix ice cold Solution A with room temperature Solution B.

---

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.