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BAM Media M154: Trypticase (Tryptic) Soy Broth

January 2001

Bacteriological Analytical Manual

Trypticase (Tryptic) Soy Broth

Trypticase peptone17 g
Phytone peptone3 g
NaCl5 g
K2HPO42.5 g
Glucose2.5 g
Distilled water1 liter


Heat with gentle agitation to dissolve. Dispense 225 ml into 500 ml Erlenmeyer flasks. Autoclave 15 min at 121°C. Final pH, 7.3 ± 0.2.


For trypticase soy broth without glucose, prepare as above, but omit 2.5 g glucose.


For use with halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.