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BAM R40: Lugol's Iodine Solution

January 2001

Bacteriological Analytical Manual

Lugol's Iodine Solution


Potassium iodide (KI) 10 g
Iodine 5 g
Distilled water 100 ml


Dissolve KI in about 20-30 ml of distilled water. Add iodine and heat gently with constant mixing until iodine is dissolved. Dilute to 100 ml with distilled water. Store in amber glass-stoppered bottle in the dark.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.