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BAM R89: Voges-Proskauer (VP) Test Reagents

January 2001

Bacteriological Analytical Manual

Voges-Proskauer (VP) Test Reagents


Solution 1


α-Naphthol5 g
Alcohol (absolute)100 ml


>Solution 2


Potassium hydroxide40 g
Distilled water to make 100 ml


Voges-Proskauer (VP) test


Transfer 1 ml of 48 h culture to test tube and add 0.6 ml solution 1 and 0.2 ml solution 2. Shake after adding each solution. To intensify and speed reaction, add a few creatine crystals to mixture. Let stand at room temperature. Read results 4 h after adding reagents.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.