Food

BAM R89: Voges-Proskauer (VP) Test Reagents

January 2001

Bacteriological Analytical Manual

R89
Voges-Proskauer (VP) Test Reagents

Solution 1

α-Naphthol 5 g
Alcohol (absolute) 100 ml

Solution 2

Potassium hydroxide 40 g
Distilled water to make 100 ml

Voges-Proskauer (VP) test

Transfer 1 ml of 48 h culture to test tube and add 0.6 ml solution 1 and 0.2 ml solution 2. Shake after adding each solution. To intensify and speed reaction, add a few creatine crystals to mixture. Let stand at room temperature. Read results 4 h after adding reagents.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

Page Last Updated: 08/03/2015
Note: If you need help accessing information in different file formats, see Instructions for Downloading Viewers and Players.