UNC TCORS: Translational Studies to Identify Epithelial Biomarkers of Smoke Exposure
Principal Investigator: Ilona Jaspers
Funding Mechanism: National Institutes of Health- TCORS Grant
ID number: 1P50HL120100-01
Award Date: 9/19/2013
Institution: University of North Carolina Chapel Hill
The respiratory epithelium is the primary target of most common respiratory viruses in humans. Cigarette smoke-induced changes in epithelial function have a significant impact on respiratory host defense and are important in determining the final outcome of infection; however, it is unknown whether tobacco alternatives (e.g., hookah, little cigars) have similar effects on epithelial host defense responses. This project will identify genomic biomarkers of smoke exposure associated with tobacco alternatives from nasal epithelial cell samples obtained from 240 adults (aged 18-45) and will evaluate respiratory host defense in subjects infected with live attenuated influenza virus. Specific aims are: (1) to use superficial nasal scrape biopsies to compare genomic and epigenomic signatures induced in epithelial cells following exposure to cigarette smoking and tobacco alternatives; (2) to use an in vitro model of differentiated human nasal epithelial cells to compare host defense responses following exposure to cigarette smoke and tobacco alternatives; and (3) to compare influenza-induced responses in cigarette smokers, hookah smokers, and e-cigarette smokers in vivo. This research will provide new information about the impact of new and emerging tobacco products on respiratory defense systems.
The Impact of Tobacco Exposure on the Lung's Innate Defense System (TCORS) Related Resources
- The Impact of Tobacco Exposure on the Lung's Innate Defense System (TCORS)
- Project 1: The Effects of New and Emerging Tobacco Products on Lung Hydration and Inflammation
- Project 2: The Effects of Tobacco Exposure on the Airway Proteome and Mucus/Mucin Integrity
- Project 3: Mouse Models of Smoking-related Diseases: What is the Best Mimic of Human Disease?
- The original scientific abstract and other project information can be found on the NIH website