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Topography, Constituents, and Toxicity of Waterpipe Tobacco Smoke Under Realistic Conditions

Principal Investigator: Irfan Rahman and Risa Robinson

Funding Mechanism: National Institutes of Health - Grant

ID number: 1R01DA042470-01

Award Date: 8/15/2016

Institution: University of Rochester

The goal of this study is to measure waterpipe smoke emissions and levels of inhaled constituents associated with different smoking topographies (use behaviors) and assess effects on pulmonary toxicity. Study aims are: (1) to compare waterpipe smoking topography and toxicity based on real-life use; (2) to identify harmful and potentially harmful constituents (HPHCs) inhaled by users under realistic use conditions based on waterpipe smoking topography; and (3) to determine the impact of waterpipe smoke on pulmonary and gene toxicity. Researchers will measure waterpipe and conventional cigarette smoking topographies (including frequency and exposure duration) in 25 waterpipe smokers, 25 cigarette smokers, and 25 dual users aged 21-65 who will use a wireless personal use monitor for three weeks; these participants and 25 nonsmokers will provide urine and blood samples to allow researchers to compare changes in biological indicators of oxidative stress and inflammatory responses. Next, waterpipe smoke emissions will be machine-generated using the subjects’ puffing topographies, and researchers will measure the chemical constituents in inhaled emissions. Finally, investigators will compare the effect of waterpipe tobacco and cigarette smoke exposures on pulmonary toxic responses (e.g., oxidative stress, inflammatory markers including lipid mediators and C-reactive protein) and gene toxicity (DNA damage/repair) in mice. Findings may inform regulatory activities related to waterpipe use.

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