Principal Investigator: Charles Murry
Funding Mechanism: National Institutes of Health- Grant
ID number: 3P01HL094374-03S1
Award Date: 9/17/2012
Institution: University of Washington
This supplement to an ongoing research project is aimed at determining the effect of smoke exposure on the differentiation of human embryonic stem cells into cardiomyocytes (hESC-CMs) and the effect that smoke exposure has on remodeling of the coronary circulation in mice. Investigators will compare the effect of exposure to smoke generated by traditional cigarettes to that generated by e-cigarettes. Specific aims are: (1) to determine the effect of traditional and e-cigarette smoke on the differentiation and function of hESC-CMs in vitro (e.g., differentiation, migration, proliferation, Ca2+ handling, excitation-contraction [EC] coupling); and (2) to determine the effect of traditional smoke exposure on the remodeling of the coronary circulation in a mouse model of myocardial infarction.