Nicotine Emissions, Toxicity, and Behavior Related to Electronic Cigarettes
Principal Investigator: Risa Robinson
Funding Mechanism: National Institutes of Health- Grant
ID number: 1R21DA036057-01
Award Date: 8/1/2013
Institution: Rochester Institute of Technology
Data describing the realistic use of electronic cigarettes (e-cigarettes) and resulting health impacts, including toxicity and abuse liability, is required. This study will compare the nicotine emissions and doses in e-cigarettes and conventional cigarettes and will evaluate whether these doses result in toxicological responses in vivo in mice. Investigators will test the hypothesis that e-cigarette users are exposed to lower amounts of nicotine than conventional cigarette users, but that these exposure levels induce toxic oxidative and inflammatory responses in vivo and will result in addiction comparable to that seen in conventional cigarette smokers. As part of the study, investigators will analyze the nicotine concentration and other constituents in e-cigarette liquid cartridges and verify levels relative to the product labels; quantify the difference in nicotine yield from a single puff of an e-cigarette versus that of a conventional cigarette for a range of machine-generated puffing topographies; compare nicotine doses between a single session of e-cigarette smoking and smoking one conventional cigarette; and compare cumulative daily doses of nicotine for e-cigarette users and conventional cigarette smokers. Specific aims are: (1) to determine how the constituents, components and design features of e-cigarettes vary by brand and how emissions are affected by consumer use behaviors; and (2) to determine if e-cigarettes cause toxic oxidative damage and pro-inflammatory effects similar to conventional cigarette smoke in vivo in mouse models, and determine the dependence potential and addictive properties of e-cigarettes in mice following chronic e-cigarette exposure.