Integrated Risk Assessment and Molecular Characterization of Pulmonary Response to E-cigarette Exposure
Principal Investigator(s): Charles K. Ansong
Funding Mechanism: NIH Grant
ID number: 1R01HL139335-01A1
Award Date: 9/13/18
Institution: Battelle Pacific Northwest Laboratories
E-cigarette aerosol is a complex mixture of e-liquid components (propylene glycol, vegetable glycerol, nicotine, water, and flavoring additives) and other constituents (such as aldehydes, metals, nanoparticles, and some unknown compounds) produced during e-cigarette heating. The goals of this study are to evaluate the oxidative stress and inflammation resulting from e-cigarette aerosol, identify aerosol constituents’ distinct “signatures” of early oxidative/nitrative damage in cells and tissue, and use these findings to evaluate the relative hazards of constituents. Researchers will use the Research Grade E-cigarette (REC) device developed at Battelle to generate and characterize aerosol from individual e-liquid components with and without the use of a heated coil. Study aims are: (1) to characterize e-cigarette aerosol generated at moderate and high heating temperatures; (2) to characterize pulmonary response to e-cigarette aerosol constituents in mice; and (3) to predict airway deposition and site-specific tissue dose and translation to humans using computational fluid dynamic-physiologically-based pharmacokinetic (CFD/PBPK) models. Study findings will provide new information about the oxidative and inflammatory effects of e-cigarette use.