High-Throughput Assay Development for Non-Nicotine Tobacco Components
Principal Investigator: Paul Whiteaker
Funding Mechanism: National Institutes of Health- Grant
ID number: 1R21DA036059-01
Award Date: 6/1/2013
Institution: St. Joseph's Hospital and Medical Center
Investigators will develop a pair of high throughput screening (HTS) assays suitable for identifying non-nicotine tobacco product compounds with activity at α3β4 and α4β2nicotinic receptors. Specific aims are: (1) to establish and optimize HTS-ready assays for a pair of existing, highly-functional, monoclonal cell lines; and (2) to configure the optimized assays for HTS conditions. This project will yield a set of validated assays that target nicotinic acetylcholine receptor subtypes tied to the use of and dependence on tobacco products and that can be used to rapidly test the large range of compounds found in existing and emerging tobacco products.