Principal Investigator: Prudence Talbot
Funding Mechanism: National Institutes of Health - Grant
ID number: 3 R01 DA036493-02S1
Award Date: 8/13/2015
Institution: University of California Riverside
The effects of heating flavor chemicals in e-cigarette liquids on human health are largely unknown. The goals of this project are to identify and quantify the reaction products formed during heating of flavored e-cigarette liquids and to determine which tests can best identify the flavor chemicals and reaction products that are harmful to the human respiratory system. Specific aims are: (1) to identify and quantify the primary flavor chemicals and major flavor-derived pyrolysis/oxidation products formed during use of approximately 100 e-cigarette refill fluids and disposable e-cigarettes; (2) to screen individual chemicals identified in Aim 1 in unheated e-cigarette fluids and aerosol condensates for toxic effects in human respiratory system cells; and (3) to test the most toxic chemicals identified in Aim 2 using air-liquid interface (ALI) technology. Researchers will conduct the experiments using a variety of methods, including gas chromatography/mass spectrometry (GC/MS), two-dimensional GC with MS, liquid chromatography/MS/MS, a moderate throughput (MTT) assay, and an ALI system that resembles a human lung. Study findings may inform regulatory activities related to flavorings in e-cigarettes.