Evaluation of Toxic Profiles of Tobacco Additives in a Systemic, Tiered Approach
Principal Investigator: Nicole Sawyer and Berran Yucesoy
Funding Mechanism: Research Contract
ID number: HHSF223201510001I
Award Date: 3/2/2016
The goal of this study is to investigate the effects of cigarette additives on in vitro mutagenicity and cytotoxicity using respiratory tract and heart cells. Investigators will first conduct a comprehensive systematic literature review to identify information on the selected additives (in unburnt and burnt form). Next, investigators will expose human airway epithelial and cardiac cell lines to freshly-generated whole cigarette smoke (using the ISO and Health Canada Intensive smoking regimens) for various assays at the air-liquid interface. Study objectives are: (1) to determine the cytotoxic potential of ingredients and/or their pyrolysis products using the neutral red assay, the lactate dehydrogenase leakage assay, and the methyl tetrazolium assay; and (2) to determine the genotoxic potential of ingredients and/or their pyrolysis products using the comet assay, Ames test, and micronucleus test.