U.S. flag An official website of the United States government
  1. Home
  2. Tobacco Products
  3. Tobacco Science & Research
  4. Research
  5. Evaluating the Toxicity and Inflammation Produced by Cigarette Smoke using Human In Vitro Airway Models
  1. Research

Evaluating the Toxicity and Inflammation Produced by Cigarette Smoke using Human In Vitro Airway Models

Principal Investigator: Xuefei Cao and Sheila M. Healy

Funding Mechanism: NCTR

ID number: C13030/E0754901

Award Date: 6/16/2014

Institution: National Center for Toxicological Research


Traditionally, tobacco smoke toxicity has been evaluated with cell cultures grown on plastic surfaces using laboratory tests that are not specifically designed to measure smoke toxicity in the human respiratory system. In recent years, air-liquid-interface (ALI) airway tissue models derived from human bronchial epithelial cells have been developed that have many of the complex structural and functional properties of human airway epithelium. In this study, researchers will use a human airway ALI tissue model to evaluate the toxicity of cigarette smoke generated from University of Kentucky reference cigarettes using both the International Organization for Standardization (ISO) and Canadian Intense (CI) smoking machine protocols. The goal is to use an in vitro system having properties similar to the intact human airway to assess and compare the adverse respiratory health effects of tobacco smoke produced under different smoking conditions. The resulting data on airway-related outcomes may inform future regulatory actions related to products claimed to be equivalent or “modified risk.”