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Development of a Standard Guideline Protocol for Rodent Tobacco Smoke Inhalation Studies

Principal Investigators: Jacob McDonald and Lynn Crosby

Funding Mechanism: Research Contract

ID number: HHSF223201510032I / HHSF22301002T

Award Date: 9/6/2016

Institution: Lovelace Biomedical and Environmental Research Institute


This study will measure the dose of cigarette smoke that represents the maximum tolerated dose (MTD), dose limiting toxicity, NOAEL (no observable effect level), and LOAEL (lowest observable effect level), as well as smoke’s effects on clinical symptoms and tissue changes at these levels.  Researchers will expose groups of 10 male and female rats for 14 days to increasing amounts of smoke from the leading brand of cigarette until the MTD is reached. Next, researchers will expose control, low, mid- and high-dose exposure groups of 10 male and female rats each to commercial cigarette smoke generated under the ISO or Canadian intense regimen for 13 weeks at the MTD, the NOAEL or LOAEL, and an intermediate dosage. Researchers will measure clinical, blood, chemistry, toxicological, and respiratory effects. This study will serve as a guideline study against which other previously-published studies can be compared in the future and will provide new data about tobacco smoke toxicity in rats.