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Development of Methods to Expose Cells in Culture to Volatile Chemicals

Principal Investigator: Xiaoqing Guo and Carmine Leggett

Funding Mechanism:  Internal FDA

ID number: E07543.01

Award Date: 8/20/2014

Institution: National Center for Toxicological Research (NCTR)

Tobacco smoke is a mixture of more than 7,000 chemicals, and more than 70 of them have been classified as carcinogens by the International Agency for Research on Cancer (IARC). Airborne particles and volatile chemicals from tobacco smoke have harmful effects on public health; as a result, it would be useful to develop standardized analytical procedures that can be used to evaluate the cell toxicity and gene toxicity potential of these chemicals. The goal of this study is to develop and demonstrate the reproducibility of a cell culture exposure protocol for aerosols and volatile chemicals. Study aims are: (1) to develop a test procedure for the mouse lymphoma assay (MLA) using cultured suspension cells, a smoking machine, exposure chambers, and whole cigarette smoke as the test material; and (2) to demonstrate the reproducibility of the MLA exposure conditions by conducting exposures with different concentrations of whole smoke over a period of six months.  The standard gene toxicity assay procedures will generate data to help determine the risks associated with exposure to aerosols and volatile chemicals in tobacco smoke.

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