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Constituent Yields and Biomarkers of Exposure for Tobacco Product Regulation

Principal Investigator: Irina Stepanov

Funding Mechanism: National Institutes of Health- Grant

ID number: 1R01CA179246-01

Award Date: 9/2/2013

Institution: University of Minnesota

The issue of how to test and regulate the contents of cigarette smoke represents a critical challenge; currently-used standard machine testing methods do not account for the complexities of smoker-cigarette interaction and are widely recognized to be inadequate for the prediction of human exposures.  This project will analyze carcinogenic tobacco-specific nitrosamines (TSNA) per mg nicotine emissions in currently marketed U.S. cigarettes; investigate the effect of different smoking machine regimens on these emissions; investigate how smoking cigarettes that differ in TSNA yield affects smoker uptake of these carcinogens; and explore a novel approach to the assessment of TSNA per mg nicotine intake by analyzing mouth-level exposure to these carcinogens based on an analysis of cigarette filters. The goal of this project is to develop a testing approach that can produce meaningful predictions of changes in human exposure due to changes in smoke constituent levels, and hence serve as a reliable measure for product regulation. Specific aims are: (1) to analyze N-nitrosonornicotine (NNN) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) per mg nicotine emissions in cigarettes representing 11 U.S. cigarette brands using different smoking machine regimens; (2) to examine the extent to which differences in smoke yields of NNN and NNK per mg nicotine predict differences in smoker exposure to these carcinogens by analyzing the urine of 300 adult smokers (aged 18-65); and (3) to determine which individual factors (e.g., duration and intensity of smoking, nicotine metabolism, demographics) may affect the relationship between machine-measured TSNA per mg nicotine and smoker exposures.

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