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Assessment of Tobacco Product Pharmacology and Behavior using Nonclinical Models

Principal Investigator: Jenny Wiley, Kia Jackson, and Steven Meredith

Funding Mechanism: Research Contract

ID number: HHSF223201310034I

Award Date: 7/25/2016

Institution: RTI International


Nicotine is as the primary constituent in tobacco that promotes addiction; however, studies indicate that minor tobacco alkaloids contribute to the reinforcing effects of nicotine. Researchers will conduct behavioral studies in adult rats to compare the addictive potential of whole tobacco extracts from various tobacco products. A smoking machine will generate whole tobacco smoke or aerosol condensate from up to 15 commercially-available U.S. brands of each of the following four tobacco products (including flavored products): cigarettes, little cigars, e-cigarettes, and waterpipe tobacco. Researchers will analyze amounts of nicotine and minor tobacco alkaloids (nornicotine, acetaldehyde, anabasine, anatabine, myosmine, cotinine, harmane, and norharmane) in the different tobacco products. After analysis, researchers will select a representative brand for each tobacco product and generate an aqueous solution for use in rodent self-administration studies. Adult male rats (4 groups with 10 rats per group) will be trained in a self-administration model designed to compare the reinforcing effects of nicotine alone (in a control solution that contains none of the minor tobacco alkaloids) and the aqueous smoke or aerosol solutions generated from each of the four products. Study results will provide information on the levels and associated abuse liability of non-nicotine tobacco constituents in several brands of different tobacco products.