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Serum-free Medium for In Vitro Isolation and Expansion of Canine Adipose-Derived Mesenchymal Stem/Stromal Cells (MSCs)

Serum-free Medium for In Vitro Isolation and Expansion of Canine Adipose-Derived Mesenchymal Stem/Stromal Cells (MSCs)

The invention offered for license is in the field of veterinary medicine.  Specifically, it relates to a novel, serum-free medium used to isolate and expand MSCs.

MSCs are a potentially effective therapy for treating a variety of conditions in veterinary medicine.  They are isolated and expanded in vitro in a medium that is supplemented with fetal bovine serum (FBS).  However, the use of FBS as a medium component during manufacturing may pose several problems.  FBS can be a source of unwanted pathogens.  The serum can introduce carry-over components that may elicit an immune response.  Further, lot-to-lot variations in FBS quality may introduce inconsistencies between batches of MSCs.

This invention uses a novel FBS-free medium that supports both the isolation and expansion of MSCs from canine adipose tissue while removing the unwanted pathogens, immune response and lot-to-lot variations.

Potential Commercial Applications: Competitive Advantages:
An isolation and expansion technique for manufacturing large quantities of MSCs in a FBS-free medium The FBS-free medium removes the potential for adventitious pathogens, unwanted immune response and lot variations in MSCs during manufacture.
The MSCs cultivated in this novel medium exhibit a more uniform size and a shorter population doubling time as compared to the FBS medium.

Inventors:
Lax Devireddy (FDA)
Michael Myers (FDA)
Rudell Screven (FDA)

Intellectual Property:
FDA Reference Number E-2017-010

Licensing Contact:
Charlene Maddox, Ph.D.
FDA Technology Transfer Program
10903 New Hampshire Ave.
Building WO1, Rm 4232
Silver Spring, MD 20993
Email: Charlene.Maddox@fda.hhs.gov
Email: FDAInventionlicensing@fda.hhs.gov
Phone: 240-402-4561