BAM R28: Gel Diffusion Agar, 1.2%
|Sodium barbital||8.0 g|
|Merthiolate (crystalline)||0.1 g|
|Noble special agar (Difco)||12.0 g|
|Distilled water||1 liter|
Dissolve NaCl, sodium barbital, and merthiolate in 900 ml distilled water. Adjust pH to 7.4 with 1 N HCl and/or 1 N NaOH. Bring volume to l liter. Add Noble agar. Melt agar mixture in Arnold steamer. Filter in steamer, while hot, through 2 layers of analytical grade filter paper (e.g., No. 588, Schleicher and Schuell or equivalent). Dispense in small (15-25 ml) portions into 4 oz prescription bottles. Do not remelt more than twice.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.