BAM Media M186: Trypicase (Tryptic) Soy Broth with ferrous sulfate
Bacteriological Analytical Manual
|Trypticase peptone||17 g|
|Phytone peptone||3 g|
|Ferrous sulfate||35 mg|
|Distilled water||1.0 liter|
Heat with gentle agitation to dissolve. Dispense 225 ml into 500 ml Erlenmeyer flasks. Autoclave 15 min at 121°C. Final pH, 7.3 ± 0.2.
Hypertext Source: adapted from Chapter 5, Bacteriological Analytical Manual, 8th Edition, Revision A, 1998. Created 2000-MAR-01