BAM Media M133: Sabouraud's Dextrose Broth and Agar
Bacteriological Analytical Manual
|Polypeptone or neopeptone||10 g|
|Distilled water||1 liter|
Dissolve completely and dispense 40 ml portions into screw-cap bottles. Final pH, 5.8. Autoclave 15 min at 118-121°C. Do not exceed 121°C.
For Sabouraud's dextrose agar, prepare broth as above and add 15-20 g agar, depending on gel strength desired. Final pH, 5.6 ± 0.2. Dispense into tubes for slants and bottles or flasks for pouring plates. Autoclave 15 min at 118-121 °C.
Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.