BAM Chapter 10: Detection of Listeria monocytogenes in Foods and Environmental Samples, and Enumeration of Listeria monocytogenes in Foods

Authors: Anthony D. Hitchins (ret.) and Karen Jinneman and Yi Chen

Current Edition

April 2022: Section E.2 correction to additives amounts; Section J. clarification on enumeration homogenate preparations
February 2022: Section E.2, correction to additives amounts; Section J, clarification on enumeration homogenate preparations; editing of other sections.
March 2017: Addition of the sample matrix for environmental samples.
January 2016: More specific sample preparation and analytical set-up instructions for qualitative detection or quantitative enumeration.
January 2016: Reorganization and editing of all sections.
February 2013: update to Table 1; update for BAM Media M52: buffered Listeria enrichment broth (BLEB) in the Media section.
November 2011: Addition of PCR confirmation for Listeria monocytogenes and Listeria spp. isolates other than L. grayi.
April 2011: Section E. Diagram describing the Henry Optical System for examination of colonies added; references for Listeria monocytogenes Risk Assessment and Guidance updated.
August 2002: Section J. Enumeration: Added instructions for positive result on all MPN tubes.
April 2001: Section H. CAMP test: Updated address for ATCC and added a link to its web page.