Food

Equivalent Testing Methodology for Agricultural Water

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FDA has determined that the following methods are scientifically valid and at least equivalent to the method of analysis in § 112.151(a), “Method 1603: Escherichia coli (E. coli) in Water by Membrane Filtration Using Modified membrane-Thermotolerant Escherichia coli Agar (Modified mTEC)” (December 2009), in accuracy, precision, and sensitivity in quantifying generic Escherichia coli in agricultural water.

  1. Method 1603: Escherichia coli (E. coli) in Water by Membrane Filtration Using Modified membrane-Thermotolerant Escherichia coli Agar (Modified mTEC) (September 2014). U.S. Environmental Protection Agency. EPA-821-R-14-010.
  2. Method 1103.1: Escherichia coli (E. coli) in Water by Membrane Filtration Using membrane-Thermotolerant Escherichia coli Agar (mTEC) (March 2010). U.S. Environmental Protection Agency. EPA-821-R-10-002.
  3. Method 1604: Total Coliforms and Escherichia coli in Water by Membrane Filtration Using a Simultaneous Detection Technique (MI Medium) (September 2002). U.S. Environmental Protection Agency. EPA-821-R-02-024.
  4. 9213 D – Natural Bathing Beaches (2007). In: Standard Methods for the Examination of Water and Wastewater, 22nd Edition (Rice E.W., et al., Ed.), 9-46 – 9-48. Washington, DC: American Public Health Association. (2012).
  5. 9222 B – Standard Total Coliform Membrane Filter Procedure (1997), followed by 9222 G – MF Partition Procedures (1997) using NA-MUG media. In: Standard Methods for the Examination of Water and Wastewater, 21st Edition (Eaton A.D., et al., Ed.), 9-60 – 9-65, and 9-70 – 9-71, respectively. Washington, DC: American Public Health Association. (2005).
  6. D 5392-93 – Standard Test Method for Isolation and Enumeration of Escherichia coli in Water by the Two-Step Membrane Filter Procedure. In: Annual Book of ASTM Standards, Volume 11.02. ASTM International. (1996, 1999, 2000).
  7. Hach Method 10029 for Coliforms – Total and E. coli disclaimer icon, using m-ColiBlue24 Broth PourRite Ampules.
  8. IDEXX Colilert Test Kit disclaimer icon, but only if using IDEXX Quanti-Tray/2000 for quantification.
  9. IDEXX Colilert-18 Test Kit disclaimer icon, but only if using IDEXX Quanti-Tray/2000 for quantification.

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Page Last Updated: 11/02/2017
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