BAM Media M185: Malt Agar (MA)

January 2001

Bacteriological Analytical Manual

Malt extract, powdered 20.0 g
Agar 20.0 g
Distilled water 1.0 liter

Mix ingredients, steam to dissolve agar and sterilize for 15 min at 121°C. Temper medium to 45°C and pour plates under aseptic conditions.

To prepare slants dispense 5-6 ml of steamed medium (before autoclaving) into each of several 16 × 125 mm screw-cap tubes, loosely cap tubes and sterilize as above. After autoclaving lay tubes in a slanting position and let them cool.

This medium is recommended as a general maintenance medium.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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