Bacteriological Analytical Manual
|Fresh eggs, yolks only||2 or 3|
|Liver veal agar||48.5 g|
|Distilled water||500 ml|
Heat with agitation to dissolve. Autoclave 15 min at 121°C. Cool to 50°C.
Egg yolk emulsion
To each 500 ml of melted liver veal agar, add 40 ml egg yolk-saline suspension (see M51 for instructions). Mix thoroughly and pour into sterile 15 × 100 mm petri dishes. Dry plates at room temperature for 2 days or at 35°C for 24 h. Check plates for sterility and store sterile plates in refrigerator. In certain instances the medium may be used without addition of egg yolk emulsion.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.