BAM Media M84: Liver-Veal-Egg Yolk Agar

January 2001

Bacteriological Analytical Manual

Fresh eggs, yolks only 2 or 3
Liver veal agar 48.5 g
Distilled water 500 ml

Heat with agitation to dissolve. Autoclave 15 min at 121°C. Cool to 50°C.

Egg yolk emulsion

To each 500 ml of melted liver veal agar, add 40 ml egg yolk-saline suspension (see M51 for instructions). Mix thoroughly and pour into sterile 15 × 100 mm petri dishes. Dry plates at room temperature for 2 days or at 35°C for 24 h. Check plates for sterility and store sterile plates in refrigerator. In certain instances the medium may be used without addition of egg yolk emulsion.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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