BAM Media M150: Trypticase Novobiocin (TN) Broth

January 2001

Bacteriological Analytical Manual

Trypticase soy broth 30 g
Bile salts No. 3 1.5 g
Dipotassium phosphate 1.5 g
Novobiocin 20 mg
Distilled water 1 liter

Dissolve all ingredients except novobiocin by heating and stirring; autoclave at 121°C for 15 min. Prepare stock solution of novobiocin by adding 20 mg monosodium novobiocin per ml of distilled water. Filter-sterilize.

Make fresh stock each time of use, or store frozen at -10°C in the dark (compound is light-sensitive) for not more than 1 month (half-life is several months at 4°C). Add 1 ml stock solution per liter of medium.

Make up fresh
Storage: Frozen [-10°C]
dark conditions
up to 1 month

Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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