BAM Media M146: Thioglycollate Medium (Fluid) (FTG)

January 2001

Bacteriological Analytical Manual

L-Cystine 0.5 g
Agar (granulated) 0.75 g
NaCl 2.5 g
Dextrose 5 g
Yeast extract 5 g
Tryptone 15 g
Sodium thioglycollate or
thioglycollic acid
0.5 g
sodium solution (1:1000), fresh
1 ml
Distilled water 1 liter

Mix L-cystine, NaCl, dextrose, yeast extract, and tryptone with 1 liter water. Heat in Arnold steamer or water bath until ingredients are dissolved. Dissolve sodium thioglycollate or thioglycollic acid in solution and adjust pH so that value after sterilization is 7.1 ± 0.2. Add sodium resazurin solution, mix, and autoclave 20 min at 121°C. If commercial media are used, dispense 10 ml portions to 16 × 150 mm tubes and autoclave 15 min at 121°C.

Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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