BAM Media M145: Tetrathionate (TT) Broth

January 2001

Bacteriological Analytical Manual

Tetrathionate broth base

Polypeptone 5 g
Bile salts 1 g
Calcium carbonate 10 g
Sodium thiosulfate·5H2O 30 g
Distilled water 1 liter

Suspend ingredients in 1 liter distilled water, mix, and heat to boiling. DO NOT AUTOCLAVE. (Precipitate will not dissolve completely.) Cool to less than 45°C. Store at 5-8°C. Final pH, 8.4 ± 0.2.

Iodine-Potassium Iodide (I2-KI) solution

Potassium iodide 5 g
Iodine, resublimed 6 g
Distilled water, sterile 20 ml

Dissolve potassium iodide in 5 ml sterile distilled water. Add iodine and stir to dissolve. Dilute to 20 ml.

Brilliant green solution

Brilliant green dye, sterile 0.1 g
Distilled water, sterile 100 m1

On day of use, add 20 ml I2-KI solution and 10 ml brilliant green solution to 1 liter base. Resuspend precipitate by gentle agitation and aseptically dispense 10 ml portions into 20 × 150 or 16 × 150 mm sterile test tubes. Do not heat medium after addition of I2-KI and dye solutions. 

Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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