Food

BAM Media M138: Simmons Citrate Agar

January 2001

Bacteriological Analytical Manual

Sodium citrate*2 g
NaCl5 g
K2HPO41 g
NH4H2PO41 g
MgSO40.2 g
Bromthymol blue0.08 g
Agar15 g
Distilled water1 liter

Heat gently with occasional agitation. Boil 1-2 min until agar dissolves. Fill 13 × 100 or 16 × 150 mm screw-cap tubes 1/3 full. Autoclave 15 min at 121°C. Before medium solidifies, incline tubes to obtain 4-5 cm slants and 2-3 cm butts. Final pH, 6.8 ± 0.2.

*Difco does not specify waters of hydration.


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

Page Last Updated: 10/17/2017
Note: If you need help accessing information in different file formats, see Instructions for Downloading Viewers and Players.
Language Assistance Available: Español | 繁體中文 | Tiếng Việt | 한국어 | Tagalog | Русский | العربية | Kreyòl Ayisyen | Français | Polski | Português | Italiano | Deutsch | 日本語 | فارسی | English