BAM Media M118a: PALCAM Listeria Selective Agar

January 2001

Bacteriological Analytical Manual

Basal medium

Peptone 23 g
Starch 1 g
NaCl 5 g
Columbia agar 13 g
Mannitol 10 g
Ferric ammonium citrate 0.5 g
Esculin (aesculin) 0.8 g
Dextrose (glucose) 0.5 g
Lithium chloride 15.0 g
Phenol red 0.08 g
Distilled water 1000 ml

Selective agents

Polymyxin B sulfate 10 mg
Acriflavin 5 mg
Ceftazidine 20 mg
Distilled water 2 ml

To make 500 ml of medium, weigh 34.4 g basal medium powder (all ingredients except the three selective agents) and suspend in 500 ml distilled water. Sterilize by autoclaving at 121°C for 15 min. Dissolve the selective agent supplement mixture in sterile distilled water at 17.5 mg/ml and filter sterilize. Add 1 ml selective agent supplement solution to 500 ml sterile basal medium that has been cooled to 50°C. Mix gently and pour plates. Final pH, 7.2 ± 0.1.

*NOTE: The basal medium powder and lyophilized supplement mixture are available commercially; follow the manufacturer's instructions for preparing the medium.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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