BAM Media M19: Bismuth Sulfite Agar (Wilson and Blair)

January 2001

Bacteriological Analytical Manual

Bismuth Sulfite Agar (Wilson and Blair)

Polypeptone (or peptone) 10 g
Beef extract 5 g
Dextrose 5 g
Na2HPO4 (anhydrous) 4 g
FeSO4 (anhydrous) 0.3 g
Bismuth sulfite (indicator) 8 g
Brilliant green 0.025 g
Agar 20 g
Distilled water 1 liter

Mix thoroughly and heat with agitation. Boil about 1 min to obtain uniform suspension. (Precipitate will not dissolve.) Cool to 45-50°C. Suspend precipitate by gentle agitation, and pour 20 ml portions into sterile 15 × 100 mm petri dishes. Let plates dry about 2 h with lids partially removed; then close plates. Final pH, 7.7 ± 0.2

DO NOT AUTOCLAVE. Prepare plates on day before streaking and store in dark. Selectivity decreases in 48h.

PREPARATION Day before use.
Loss of Selectivity after 48 h

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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