BAM Media M188a: Universal Preenrichment Broth (without ferric ammonium citrate)

December 2007

Bacteriological Analytical Manual

Tryptone 5 g
Proteose peptone 5 g
KH2PO4 15 g
Na2HPO4 7 g
NaCl 5 g
Dextrose 0.5 g
MgSO4 0.25 g
Sodium pyruvate 0.2 g
Distilled water 1.0 liter

Heat with gentle agitation to dissolve. Autoclave 15 min at 121°C. Final pH, 6.3 ± 0.2.

Chemical Name: potassium dihydrogen phosphate (KH2PO4)
IUPAC International Chemical Identifier: InChI=1/K.H3O4P/c;1-5(2,3)4/h;(H3,1,2,3,4)/q+1;/p-1

Chemical Name: disodium hydrogen phosphate (Na2HPO4)
IUPAC International Chemical Identifier: InChI=1/2Na.H3O4P/c;;1-5(2,3)4/h;;(H3,1,2,3,4)/q2*+1;/p-2

Chemical Name: sodium chloride (NaCl)
IUPAC International Chemical Identifier: InChI=1/ClH.Na/h1H;/q;+1/p-1

Chemical Name: dextrose
IUPAC International Chemical Identifier: InChI=1/C6H12O6/c7-1-2-3(8)4(9)5(10)6(11)12-2/h2-11H,1H2/t2-,3-,4+,5-,6?/m1/s1

Chemical Name: magnesium sulfate (MgSO4)
IUPAC International Chemical Identifier: InChI=1/Mg.H2O4S/c;1-5(2,3)4/h;(H2,1,2,3,4)/q+2;/p-2

Chemical Name: sodium pyruvate
IUPAC International Chemical Identifier: InChI=1/C3H4O3.Na/c1-2(4)3(5)6;/h1H3,(H,5,6);/q;+1/p-1


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