BAM Media M169: Tryptose-Sulfite-Cycloserine (TSC) Agar

January 2001

Bacteriological Analytical Manual

Tryptose-Sulfite-Cycloserine (TSC) Agar

Tryptose 15 g
Yeast extract 5 g
Soytone 5 g
Ferric ammonium citrate
(NF Brown Pearls)
1 g
Sodium metabisulfite 1 g
Agar 20 g
Distilled water 900 ml

Heat with agitation to dissolve. Adjust pH to 7.6 ± 0.2. Dispense 250 ml portions to 500 ml flasks. Autoclave 15 min at 121°C. Maintain medium at 50°C before use. Dehydrated SFP agar base (Difco) is satisfactory for base.

D-cycloserine solution

Dissolve 1 g D-cycloserine (white crystalline powder) in 200 ml of distilled water. Sterilize by filtration and store at 4°C until use. D-cycloserine powder is available from Sigma Chemical Co., St. Louis, MO.

Final medium

For pour plates, add 20 ml of D-cycloserine solution to 250 ml base. To prepare prepoured plates containing egg yolk, also add 20 ml of 50% egg yolk emulsion (M51). Mix well and dispense 18 ml into 15 × 100 mm petri dishes. Cover plates with a towel and let dry overnight at room temperature before use.

Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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