BAM R16: Crystal Violet Stain (for Bacteria)

January 2001

Bacteriological Analytical Manual

Crystal Violet Stain (for Bacteria)

1. Crystal violet in dilute alcohol

Crystal violet (90% dye content) 2 g
Ethanol (95%) 20 ml
Distilled water 80 ml

2. Ammonium oxalate crystal violet (Hucker's [See R32])

Either solution is generally considered suitable as a simple stain to observe morphology.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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