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U.S. Department of Health and Human Services

Vaccines, Blood & Biologics

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HIV, Influenza, and Smallpox Vaccines: Development of New Assays and Animal Models for Evaluation of Vaccine Safety and Efficacy

Principal Investigator: H. Golding, PhD
Office / Division / Lab: OVRR / DVP / LR


General Overview

Industry and academic researchers are developing new vaccines against viruses, including HIV, pandemic influenza strains of avian origin(H5N1, H7N7, H7N9, H9N9), respiratory syncyia virus (RSV), Ebola, smallpox and the Zika virus. Novel "Universal Influenza Vaccines" are also under development. Many of these vaccines are being developed and tested together with novel adjuvants (agents that stimulate or increase immune response to vaccines).

Therefore, in addition to evaluating the safety and efficacy of the vaccine itself, FDA must also evaluate the combination of vaccine and its adjuvant. Moreover, evaluation of each viral vaccine presents unique challenges that agency regulators must address individually.

The goal of our program is to develop new and improved tools for monitoring vaccine safety and efficacy. FDA will then share these new tools with the regulated industry to enable use of these tools in their research and development efforts.

The availability of new tools could reduce the time it takes to develop new vaccines against HIV, influenza, RSV, Ebola, smallpox, and Zika and help FDA to recognize quickly potential safety problems with vaccines that use new adjuvants. Thus, our work will contribute significantly to public health in the US and globally by facilitating development and approval of new vaccines that prevent serious diseases.


Scientific Overview

Our laboratory is working to generate effective vaccines against highly pathogenic avian influenza (H5, H7 H7, H9, H2) based on the globular head of the influenza hemagglutinin (HA) form the corresponding viruses.We are also exploring new protective targets in RSV, Ebola, and in the future, Zika virus.

Future vaccines for influenza strains with pandemic potential will likely be combined with adjuvants in order to improve their immunogenicity, to generate heterosubtypic neutralizing activity, and to reduce the amount of antigen required for vaccination--an important advantage for global vaccination campaigns.

In parallel with vaccine development, we are working to improve the analytical tools available for comparing immune responses generated by different vaccine candidates. Our laboratory initiated the use of whole genome phage display libraries (GFPDL)to screen antibody repertoires, and surface plasmon resonance to measure antibody binding to properly folded viral surface proteins and to measure antibody affinity maturation. These tools are used to screen convalescent sera, monoclonal antibodies, and to compare immune sera from recipients of unadjuvanted vaccines with that of recipients of adjuvanted vaccines.

The same tools have been developed for RSV, which causes high morbidity in newborn children and in frail old individuals. New RSV vaccines are in advance development to be evaluated in the very old, the very young (newborn infants) and pregnant mothers.

The Ebola pandemic in West Africa required concerted efforts to stop the outbreaks in several countries and to rapidly develope and test new vaccines, which are currently being evlauted in clinical trials. We are developing novel methods to better explore the antibody reperoires following Ebola infection and vaccination.

The new analytical tools developed in our lab will help to find the correlates of protection against influenza, RSV, Ebola (and Zika), could contribute to the design of improved vaccines with broad cross-protection potentital.

Some new adjuvants contain components that can cause unacceptable reactogenicity and systemic adverse reactions. Often these adverse reactions are undetectable in pre-clinical toxicity studies in small animals due to species differences in the cellular receptor targeted by the novel adjuvants or because of their lower sensitivity to drug-associated toxicities. Therefore, we are developing rapid in vitro screening assays based on human cells to evaluate the activity of new adjuvants and to identify parameters that will predict unacceptable toxicities including fever in humans.

Our program is also continuing in the application of whole-body bioimaging of animals challenged with recombinant vaccinia-luciferase. This new approach, in addition to the use of advanced statistic tools, provides a practical, quantitative, and humane approach to following virus dissemination in animal models and for evaluating the efficacy of novel vaccines and therapies for smallpox.


Publications

Antiviral Res 2017 Aug;144:8-20
Development of an animal model of progressive vaccinia in nu/nu mice and the use of bioluminescence imaging for assessment of the efficacy of monoclonal antibodies against vaccinial B5 and L1 proteins.
Zaitseva M, Thomas A, Meseda CA, Cheung CYK, Diaz CG, Xiang Y, Crotty S, Golding H

J Transl Med 2017 Jul 10;15(1):155
Impaired B cell immunity in acute myeloid leukemia patients after chemotherapy.
Goswami M, Prince G, Biancotto A, Moir S, Kardava L, Santich BH, Cheung F, Kotliarov Y, Chen J, Shi R, Zhou H, Golding H, Manischewitz J, King L, Kunz LM, Noonan K, Borrello IM, Smith BD, Hourigan CS

Clin Vaccine Immunol 2017 Mar 6;24(3):e00498-16
Preexisting immunity not frailty phenotype predicts influenza post vaccination titers among older veterans.
Van Epps P, Tumpey T, Pearce MB, Golding H, Higgins P, Hornick T, Burant C, Wilson BM, Banks R, Gravenstein S, Canaday DH

Sci Rep 2017 Feb 10;7:42428
Preclinical evaluation of bacterially produced RSV-G protein vaccine: Strong protection against RSV challenge in cotton rat model.
Fuentes S, Klenow L, Golding H, Khurana S

Vaccine 2017 Jan 23;35(4):694-702
Development of bioluminescence imaging of respiratory syncytial virus (RSV) in virus-infected live mice and its use for evaluation of therapeutics and vaccines.
Fuentes S, Arenas D, Moore MM, Golding H, Khurana S

Nat Med 2016 Dec;22(12):1439-47
Human antibody repertoire after VSV-Ebola vaccination identifies novel targets and virus-neutralizing IgM antibodies.
Khurana S, Fuentes S, Coyle EM, Ravichandran S, Davey RT Jr, Beigel JH

J Virol 2016 Sep 29;90(20):9383-93
Antigenic fingerprinting of antibody response following highly pathogenic H7N7 avian influenza virus exposure in humans: Evidence for anti-PA-X antibodies.
Khurana S, Chung KY, Coyle EM, Meijer A, Golding H

Sci Rep 2016 May 27;6:26494
ICOS(+)PD-1(+)CXCR3(+) T follicular helper cells contribute to the generation of high-avidity antibodies following influenza vaccination.
Bentebibel SE, Khurana S, Schmitt N, Kurup P, Mueller C, Obermoser G, Palucka AK, Albrecht RA, Garcia-Sastre A, Golding H, Ueno H

Sci Rep 2016 Apr 25;6:24897
Production of potent fully human polyclonal antibodies against Ebola Zaire virus in transchromosomal cattle.
Dye JM, Wu H, Hooper JW, Khurana S, Kuehne AI, Coyle EM, Ortiz RA, Fuentes S, Herbert AS, Golding H, Bakken RA, Brannan JM, Kwilas SA, Sullivan EJ, Luke TC, Smith G, Glenn G, Li W, Ye L, Yang C, Compans RW, Tripp RA, Jiao JA

PLoS Pathog 2016 Apr 21;12(4):e1005554
Antigenic fingerprinting following primary RSV infection in young children identifies novel antigenic sites and reveals unlinked evolution of human antibody repertoires to fusion and attachment glycoproteins.
Fuentes S, Coyle EM, Beeler J, Golding H, Khurana S

Neurol Neuroimmunol Neuroinflamm 2016 Jan 27;3(1):e196
Patients with MS under daclizumab therapy mount normal immune responses to influenza vaccination.
Lin YC, Winokur P, Blake A, Wu T, Manischewitz J, King LR, Romm E, Golding H, Bielekova B

J Infect Dis 2015 Oct 15;212(8):1270-8
High affinity H7 head and stalk domain-specific antibody responses to an inactivated influenza H7N7 vaccine after priming with live attenuated influenza vaccine.
Halliley JL, Khurana S, Krammer F, Fitzgerald T, Coyle EM, Chung KY, Baker SF, Yang H, Martínez-Sobrido L, Treanor JJ, Subbarao K, Golding H, Topham DJ, Sangster MY

MBio 2015 Aug 4;6(4):e01156-15
A simple flow-cytometric method measuring B cell surface immunoglobulin avidity enables characterization of affinity maturation to influenza A virus.
Frank GM, Angeletti D, Ince WL, Gibbs JS, Khurana S, Wheatley AK, Max EE, McDermott AB, Golding H, Stevens J, Bennink JR, Yewdell JW

J Virol 2015 Mar 15;89(6):3295-307
Post-challenge administration of Brincidofovir protects normal and immune-deficient mice reconstituted with limited numbers of T cells from lethal challenge with IHD-J-Luc vaccinia virus.
Zaitseva M, McCullough KT, Cruz S, Thomas A, Diaz CG, Keilholz L, Grossi IM, Trost LC, Golding H

PLoS One 2015 Jan 28;10(1):e0115476
Oral priming with replicating adenovirus serotype 4 followed by subunit H5N1 vaccine boost promotes antibody affinity maturation and expands H5N1 cross-clade neutralization.
Khurana S, Coyle EM, Manischewitz J, King LR, Ishioka G, Alexander J, Smith J, Gurwith M, Golding H

Virology 2014 Oct 28;471-3:93-104
Live attenuated influenza A virus vaccine protects against A(H1N1)pdm09 heterologous challenge without vaccine associated enhanced respiratory disease.
Gauger PC, Loving CL, Khurana S, Lorusso A, Perez DR, Kehrli ME Jr, Roth JA, Golding H, Vincent AL

J Infect Dis 2014 Jun 15;209(12):1860-9
A live attenuated influenza A(H5N1) vaccine induces long-term immunity in the absence of a primary antibody response.
Talaat KR, Luke CJ, Khurana S, Manischewitz J, King LR, McMahon BA, Karron RA, Lewis KD, Qin J, Follmann DA, Golding H, Neuzil KM, Subbarao K

Cell 2014 Apr 10;157(2):499-513
Global analyses of human immune variation reveal baseline predictors of postvaccination responses.
Tsang JS, Schwartzberg PL, Kotliarov Y, Biancotto A, Xie Z, Germain RN, Wang E, Olnes MJ, Narayanan M, Golding H, Moir S, Dickler HB, Perl S, Cheung F, Baylor HIPC Center, CHI Consortium

PLoS One 2014 Apr 22;9(4):e95496
Heterologous prime-boost vaccination with MF59-adjuvanted H5 vaccines promotes antibody affinity maturation towards the hemagglutinin HA1 domain and broad H5N1 cross-clade neutralization.
Khurana S, Coyle EM, Dimitrova M, Castellino F, Nicholson K, Giudice GD, Golding H

Vaccine 2014 Apr 17;32(19):2188-97
Novel antibody-independent receptor-binding SPR-based assay for rapid measurement of influenza vaccine potency.
Khurana S, King LR, Manischewitz J, Coyle EM, Golding H

Biologicals 2013 Nov;41(6):458-68
Predictive markers of safety and immunogenicity of adjuvanted vaccines.
Mastelic B, Garcon N, Del Giudice G, Golding H, Gruber M, Neels P, Fritzell B

J Virol 2013 Sep;87(17):9742-53
Influenza a virus hemagglutinin trimerization completes monomer folding and antigenicity.
Magadán JG, Khurana S, Das SR, Frank GM, Stevens J, Golding H, Bennink JR, Yewdell JW

J Infect Dis 2013 Aug;208(3):413-7
DNA priming prior to H5N1 inactivated influenza vaccination expands antibody epitope repertoire and increases affinity maturation in a boost-interval-dependent manner in adults.
Khurana S, Wu J, Dimitrova M, King LR, Manischewitz J, Graham BS, Ledgerwood JE, Golding H

Vaccine 2013 Aug 20;31(37):3987-94
Development of a simple, rapid, sensitive, high-throughput luciferase reporter based microneutralization test for measurement of virus neutralizing antibodies following respiratory syncytial virus vaccination and infection.
Fuentes S, Crim RL, Beeler J, Teng MN, Golding H, Khurana S

Sci Transl Med 2013 Aug 28;5(200):200ra114
Vaccine-induced anti-HA2 antibodies promote virus fusion and enhance influenza virus respiratory disease.
Khurana S, Loving CL, Manischewitz J, King LR, Gauger PC, Henningson J, Vincent AL, Golding H

Vaccine 2013 Jul 11;31(32):3209-21
Strengthening the influenza vaccine virus selection and development process: Outcome of the 2nd WHO Informal Consultation for Improving Influenza Vaccine Virus Selection held at the Centre International de Conferences (CICG) Geneva, Switzerland, 7 to 9 December 2011.
Al Busaidy S, Al-Gohary A, Ampofo WK, Bekshin Z, Cerkaoui I, Claes F, Cox NJ, Cracknell W, Cui L, Paiva TM, de Wit M, Diep LDT, Donabedian A, Donis R, Dormitzer PR, Eichelberger M, El Kholy N, Engelhardt O, Engelmann HB, Eropkin MY, Fasce R, Fontaine L, Ghadakchi HF, Ghedin E, Giovanni MY, Golding H, da Silva HC, Gysin R, Hamilton K, Hauge SH, Hay A, Huang S, Hungnes O, Ibrahim SSS, Immamura T, Inglis SC, Kadjo H, Katz J, Kawaoka Y, Kelso A, Khan MI, Kilander A, Klimov A, Kreij K, Kusmiaty D, Linde A, Liu MT, Lupulescu E, Mai LQ, Martinez VR, McCauley J, Meyer Q, Modi R, Mohammadi AA, Mokhtari-Azad T, Mol E, Music T, Nelson K, Neuhaus E, Neumeier ER, Niang MN, Odagiri T, Peiris M, Pohlmann A, Popow-Kraupp T, Trilar KP, Rabadan R, Rambaut A, Robertson JS, Rolls C, Sampurno OD, Savy V, Sawanpanyaltert P, Schmid G, Schweiger B, Shapira S, Shinde V, Shu Y, Siqueira M, Smith D, Spiro D, Stevens J, Takizawa K, Tashiro M, Taylor B, Thomas Y, Tsai T, Van der Werf S, Venter M, Waddell AL, Wan XF, Wang D, Webby R, Weir JP, Wentworth D, Wickramasinghe G, Ye ZP, Zambon M, Ziegler T

Sci Transl Med 2013 Jun 26;5(191):191ed8
Getting to the heart of influenza.
Yewdell JW, Spiro DJ, Golding H, Quill H, Mittelman A, Nabel GJ

J Virol 2013 May;87(10):5564-76
Effects of post-challenge administration of ST-246 on dissemination of IHD-J-Luc vaccinia virus in normal and in immune deficient mice reconstituted with T cells.
Zaitseva M, Shotwell E, Scott J, Cruz S, King LR, Manischewitz J, Diaz CG, Jordan R, Grosenbach D, Golding H

Biologicals 2013 Mar;41(2):115-24
Potential use of inflammation and early immunological event biomarkers in assessing vaccine safety.
Mastelic B, Lewis DJ, Golding H, Gust I, Sheets R, Lambert PH

J Infect Dis 2013 Jan;207(2):288-96
Heterovariant Cross-Reactive B-Cell Responses Induced by the 2009 Pandemic Influenza Virus A Subtype H1N1 Vaccine.
He XS, Sasaki S, Baer J, Khurana S, Golding H, Treanor JJ, Topham DJ, Sangster MY, Jin H, Dekker CL, Subbarao K, Greenberg HB

Proc Natl Acad Sci U S A 2013 Jan 2;110(1):264-9
Preconfiguration of the antigen-binding site during affinity maturation of a broadly neutralizing influenza virus antibody.
Schmidt AG, Xu H, Khan AR, O'Donnell T, Khurana S, King LR, Manischewitz J, Golding H, Suphaphiphat P, Carfi A, Settembre EC, Dormitzer PR, Kepler TB, Zhang R, Moody MA, Haynes BF, Liao HX, Shaw DE, Harrison SC

J Virol 2012 Nov;86(22):12283-93
Oligomeric recombinant H5 HA1 vaccine produced in bacteria protects ferrets from homologous and heterologous wild type H5N1 influenza challenge and control viral loads better than subunit H5N1 vaccine by eliciting high affinity antibodies.
Verma S, Dimtrova M, Munjal A, Fontana J, Crevar CJ, Carter DM, Ross TM, Khurana S, Golding H

PLoS Pathog 2012 Sep;8(9):e1002920
AID activity in B cells strongly correlates with polyclonal antibody affinity maturation in-vivo following pandemic 2009-H1N1 vaccination in humans.
Khurana S, Frasca D, Blomberg B, Golding H

Vaccine 2012 Jul 6;30(32):4859-65
159-Use of human MonoMac6 cells for development of in vitro assay predictive of adjuvant safety in vivo.
Zaitseva M, Romantseva T, Blinova K, Beren J, Sirota L, Drane D, Golding H

Methods Mol Biol 2012;890:161-76
Measurements of vaccinia virus dissemination using whole body imaging: approaches for predicting of lethality in challenge models and testing of vaccines and antiviral treatments.
Zaitseva M, Kapnick S, Golding H

J Virol 2012 May;86(10):5515-22
Influenza virus H1N1pdm09 infections in the young and old: evidence of greater antibody diversity and affinity for the hemagglutinin globular head domain (HA1 Domain) in the elderly than in young adults and children.
Verma N, Dimitrova M, Carter DM, Crevar CJ, Ross TM, Golding H, Khurana S

J Infect Dis 2012 Feb;205(4):610-20
Immune response following H1N1pdm09 vaccination: differences in antibody repertoire and avidity in young adults and elderly populations stratified by age and gender.
Khurana S, Verma N, Talaat KR, Karron RA, Golding H

J Virol 2011 Dec;85(23):12455-63
H5N1-SeroDetect EIA and rapid test: a novel differential diagnostic assay for serodiagnosis of H5N1 infections and surveillance.
Khurana S, Sasono P, Fox A, Kinh NV, Mai LQ, Thai PQ, Hien NT, Liem NT, Horby P, Golding H

J Virol 2011 Nov;85(21):10945-54
H5N1 virus-like particle vaccine elicits cross-reactive neutralizing antibodies in humans that preferentially bind to oligomeric form of influenza hemagglutinin.
Khurana S, Wu J, Verma N, Verma S, Raghunandan R, Manischewitz J, King LR, Kpamegan E, Pincus S, Smith G, Glenn G, Golding H

J Virol 2011 Sep;85(17):9147-58
Passive immunotherapies protect WRvFire and IHD-J-Luc vaccinia virus infected mice from lethality by reducing viral loads in the upper respiratory tract and internal organs.
Zaitseva M, Kapnick SM, Meseda CA, Shotwell E, King LR, Manischewitz J, Scott J, Kodihalli S, Merchlinsky M, Nielsen H, Lantto J, Weir JP, Golding H

Vaccine 2011 Aug 5;29(34):5657-65
Recombinant HA1 produced in E. coli forms functional oligomers and generates strain-specific SRID potency antibodies for pandemic influenza vaccines.
Khurana S, Larkin C, Verma S, Joshi MB, Fontana J, Steven AC, King LR, Manischewitz J, McCormick W, Gupta RK, Golding H

Proc Natl Acad Sci U S A 2011 Aug 23;108(34):14216-21
Broadly neutralizing human antibody that recognizes the receptor-binding pocket of influenza virus hemagglutinin.
Whittle JR, Zhang R, Khurana S, King LR, Manischewitz J, Golding H, Dormitzer PR, Haynes BF, Walter EB, Moody MA, Kepler TB, Liao HX, Harrison SC

Sci Transl Med 2011 Jun 1;3(85):85ra48
MF59 adjuvant enhances diversity and affinity of antibody-mediated immune response to pandemic influenza vaccines.
Khurana S, Verma N, Yewdell JW, Hilbert AK, Castellino F, Lattanzi M, Del Giudice G, Rappuoli R, Golding H

J Virol 2011 Feb;85(3):1246-56
Bacterial HA1 vaccine against pandemic H5N1 influenza: evidence of oligomerization, hemagglutination, and cross- protective immunity in ferrets.
Khurana S, Verma S, Verma N, Crevar CJ, Carter DM, Manischewitz J, King LR, Ross TM, Golding H

PLoS One 2011 Feb 28;6(2):e17297
A rapid Flp-In system for expression of secreted H5N1 influenza hemagglutinin vaccine immunogen in mammalian cells.
Lu H, Khurana S, Verma N, Manischewitz J, King L, Beigel JH, Golding H

Immunol Rev 2011 Jan;239(1):167-77
Influenza vaccine immunology.
Dormitzer PR, Galli G, Castellino F, Golding H, Khurana S, Del Giudice G, Rappuoli R

FASEB J 2011 Jan;25(1):55-65
Receptor activity modifying protein-3 mediates the protumorigenic activity of lysyl oxidase-like protein-2.
Brekhman V, Lugassie J, Zaffryar-Eilot S, Sabo E, Kessler O, Smith V, Golding H, Neufeld G

PLoS One 2010 Jul 12;5(7):e11548
Properly folded bacterially expressed H1N1 hemagglutinin globular head and ectodomain vaccines protect ferrets against H1N1 pandemic influenza virus.
Khurana S, Verma S, Verma N, Crevar CJ, Carter DM, Manischewitz J, King LR, Ross TM, Golding H

Vaccine 2010 Jan 8;28(3):790-7
A practical influenza neutralization assay to simultaneously quantify hemagglutinin and neuraminidase-inhibiting antibody responses.
Hassantoufighi A, Zhang H, Sandbulte M, Gao J, Manischewitz J, King L, Golding H, Straight TM, Eichelberger MC

J Clin Microbiol 2010 Jan;48(1):281-5
HIV-Selectest enzyme immunoassay and rapid test: ability to detect seroconversion following HIV-1 infection.
Khurana S, Norris PJ, Busch MP, Haynes BF, Park S, Sasono P, Mlisana K, Salim AK, Hecht FM, Mulenga J, Chomba E, Hunter E, Allen S, Nemo G, Rodriguez-Chavez IR, Women's Interagency HIV Study, Joseph B. Margolick on behalf of Multicenter AIDS Cohort Study (MACS), Golding H

Sci Transl Med 2010 Jan 20;2(15):15ra5
Vaccines with MF59 adjuvant expand the antibody repertoire to target protective sites of pandemic avian H5N1 influenza virus.
Khurana S, Chearwae W, Castellino F, Manischewitz J, King LR, Honorkiewicz A, Rock MT, Edwards KM, Del Giudice G, Rappuoli R, Golding H

J Virol 2009 Oct;83(20):10437-47
Application of bioluminescence imaging to the prediction of lethality in vaccinia virus infected mice.
Zaitseva M, Kapnick SM, Scott J, King LR, Manischewitz J, Sirota L, Kodihalli S, Golding H

Clin Vaccine Immunol 2009 Aug;16(8):1105-12
Statistical approach to estimate vaccinia-specific neutralizing antibody titers using a high throughput assay.
Kennedy R, Pankratz VS, Swanson E, Watson D, Golding H, Poland GA

Proc Natl Acad Sci U S A 2009 Aug 17;106(35):14802-7
Conversion of MDCK cell line to suspension culture by transfecting with human siat7e gene and its application for influenza virus production.
Chu C, Lugovtsev V, Golding H, Betenbaugh M, Shiloach J

J Virol 2009 May;83(9):4624-30
Electroporation of Synthetic DNA Antigens Offers Protection in Non-Human Primates Challenged with Highly Pathogenic Avian Influenza.
Laddy DJ, Yan J, Khan AS, Anderson H, Cohn A, Greenhouse J, Lewis M, Manischewitz J, King LR, Golding H, Draghia-Akli R, Weiner DB

PLoS Med 2009 Apr 21;6(4):e1000049
Antigenic fingerprinting of H5N1 avian influenza using convalescent sera and monoclonal antibodies reveals potential vaccine and diagnostic targets.
Khurana S, Suguitan AL Jr, Rivera Y, Simmons CP, Lanzavecchia A, Sallusto F, Manischewitz J, King LR, Subbarao K, Golding H

Vaccine 2008 Aug 12;26(34):4299-303
FDA/NIH/WHO public workshop on immune correlates of protection against influenza A viruses in support of pandemic vaccine development, Bethesda, Maryland, US, December 10-11, 2007.
Eichelberger M, Golding H, Hess M, Weir J, Subbarao K, Luke CJ, Friede M, Wood D

Int Immunol 2007 Nov;19(11):1249-60
Regeneration of the adult thymus is preceded by the expansion of K5+K8+ epithelial cell progenitors and by increased expression of Trp63, cMyc, and Tcf3 transcription factors in the thymic stroma
Popa I, Zubkova I, Medvedovic M, Romantseva T, Mostowski H, Boyd R, Zaitseva M

J Infect Dis 2007 Oct 1;196(7):1026-32
Antibodies to the A27 Protein of Vaccinia Virus Neutralize and Protect against Infection but Represent a Minor Component of Dryvax Vaccine-Induced Immunity.
He Y, Manischewitz J, Meseda CA, Merchlinsky M, Vassell RA, Sirota L, Berkower I, Golding H, Weiss CD

AIDS 2007 Feb 19;21(4):521-4
Durable neutralizing antibodies after remote smallpox vaccination among adults with and without HIV infection.
Kan VL, Manischewitz J, King LR, Golding H

J Acquir Immune Defic Syndr 2006 Nov 1;43(3):304-12
Novel Approach for Differential Diagnosis of HIV Infections in the Face of Vaccine-Generated Antibodies: Utility for Detection of Diverse HIV-1 Subtypes.
Khurana S, Needham J, Park S, Mathieson B, Busch MP, Nemo G, Nyambi P, Zolla-Pazner S, Laal S, Mulenga J, Chomba E, Hunter E, Allen S, McIntyre J, Hewlett I, Lee S, Tang S, Cowan E, Beyrer C, Altfeld M, Yu XG, Tounkara A, Koita O, Kamali A, Nguyen N, Graham BS, Todd D, Mugenyi P, Anzala O, Sanders E, Ketter N, Fast P, Golding H

J Immunol 2006 Aug 15;177(4):2552-64
Subunit recombinant vaccine protects against monkeypox.
Heraud JM, Edghill-Smith Y, Ayala V, Kalisz I, Parrino J, Kalyanaraman VS, Manischewitz J, King LR, Hryniewicz A, Trindade CJ, Hassett M, Tsai WP, Venzon D, Nalca A, Vaccari M, Silvera P, Bray M, Graham BS, Golding H, Hooper JW, Franchini G

J Virol 2006 Mar;80(5):2092-9
Human Immunodeficiency Virus (HIV) Vaccine Trials: a Novel Assay for Differential Diagnosis of HIV Infections in the Face of Vaccine-Generated Antibodies.
Khurana S, Needham J, Mathieson B, Rodriguez-Chavez IR, Catanzaro AT, Bailer RT, Kim J, Polonis V, Cooper DA, Guerin J, Peterson ML, Gurwith M, Nguyen N, Graham BS, Golding H

Clin Infect Dis 2006 Feb 15;42(4):e16-20
Clinical and immunological comparison of smallpox vaccine administered to the outer versus the inner upper arms of vaccinia-naive adults.
Waibel KH, Golding H, Manischewitz J, King LR, Tuchscherer M, Topolski RL, Walsh DS

J Leukoc Biol 2005 Dec;78(6):1306-17
Increased CXCR4-dependent HIV-1 fusion in activated T cells: role of CD4/CXCR4 association.
Zaitseva M, Romantseva T, Manischewitz J, Wang J, Goucher D, Golding H

Virology 2005 Sep 1;339(2):164-75
Enhanced immunogenicity and protective effect conferred by vaccination with combinations of modified vaccinia virus Ankara and licensed smallpox vaccine Dryvax in a mouse model.
Meseda CA, Garcia AD, Kumar A, Mayer AE, Manischewitz J, King LR, Golding H, Merchlinsky M, Weir JP

J Biol Chem 2005 Aug 19;280(33):29570-7
CCR5 N-terminal region plays a critical role in HIV-1 inhibition by toxoplasma gondii derived cyclophilin-18.
Golding H, Khurana S, Yarovinsky F, King LR, Abdoulaeva G, Antonsson L, Owman C, Platt EJ, Kabat D, Andersen JF, Sher A

Nat Med 2005 Jul;11(7):740-7
Smallpox vaccine-induced antibodies are necessary and sufficient for protection against monkeypox virus.
Edghill-Smith Y, Golding H, Manischewitz J, King LR, Scott D, Bray M, Nalca A, Hooper JW, Whitehouse CA, Schmitz JE, Reimann KA, Franchini G

J Virol 2005 Jun;79(11):6791-800
Identification of a Linear Peptide Recognized by Monoclonal Antibody 2D7 Capable of Generating CCR5-Specific Antibodies with Human Immunodeficiency Virus-Neutralizing Activity.
Khurana S, Kennedy M, King LR, Golding H

Biotechnol Bioeng 2005 Jun 20;90(6):663-74
Production of recombinant proteins by vaccinia virus in a microcarrier based mammalian cell perfusion bioreactor.
Bleckwenn NA, Golding H, Bentley WE, Shiloach J

J Infect Dis 2005 Feb 1;191(3):372-81
Smallpox vaccine does not protect macaques with AIDS from a lethal monkeypox virus challenge.
Edghill-Smith Y, Bray M, Whitehouse CA, Miller D, Mucker E, Manischewitz J, King LR, Robert-Guroff M, Hryniewicz A, Venzon D, Meseda C, Weir J, Nalca A, Livingston V, Wells J, Lewis MG, Huggins J, Zwiers SH, Golding H, Franchini G.

 

 
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