About FDA

Pathology Services

On-Site Contractor Provided Services

Pathology services are provided by an on-site contractor who operates government-owned facilities at NCTR, supplying NCTR's investigators with state-of-the art services such as:

General Pathology Services
Reproduction, Development, and Gene Services
Assay Services
Tissue Sections
Imaging Services
Clinical Chemistry, Hematology and Urinalysis Tests
Coagulation Testing

General Pathology Services
  • Animal necropsy and histopathology
  • Whole body perfusion fixation of rodents and non-human primates
  • Zebrafish colony surveillance
  • Virtual microscopy/pathology system (ScanScope) allowing digital storage of an entire microscope slide at diagnostic resolution
  • Glycol methacrylate and methyl methacrylate plastic sectioning
  • Glycol methacrylate embedding and sectioning at ½ micron in thickness for examination by the light microscope
  • Gross Pathology System (GPS) for automated collection of gross pathology data coordinated with Inlife data
  • DNA or RNA isolation from microdissected material
  • Laser Capture Microdissection (LMD)
  • Immunohistochemistry (IHC) for numerous hormones, hormone receptors, oncoproteins, and other proteins as requested by investigators
  • Nonradioactive in situ hybridization (ISH)
  • PCR (solution PCR, solution RT-PCR, in situ PCR, in situ RT-PCR)
  • Plastic embedding
  • Quantum dots (nanoscale crystals) as markers of in vivo biologic phenomena
  • Recycling waste ethanol, methanol, acetone, xylene to histological grade solvents, waste formalin to 10% Neutral Buffered Formalin
  • Archive Repository for experiment residual wet tissues, paraffin embedded tissue blocks and slides
  • Maintain current inventory of Safety Data Sheets (SDS) and Hazardous Chemical Inventory

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Reproduction, Development, and Gene Services
  • Automated sperm analysis for count, motility, and morphology
  • Developmental toxicity indices (Segment I Reproduction Toxicity, Segment II Teratology, Segment III Perinatal Toxicity, fertility assessment by continuous breeding)
  • Vaginal cytology specimen processing and evaluation of reproductive cycles
  • Ovarian toxicity assessment using standardized follicle count procedures
  • Genetic monitoring of rodent strains by microsatellate DNA analysis
  • Confirmation of successful breeding by assessment of vaginal smears for presence of sperm

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Assay Services
  • Apoptosis assays (TUNEL, ISOL, Caspase-3 IHC)
  • Proliferation assays (BrdU, Ki67, PNCA and ISH for Histone mRNA)

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Tissue Sections
  • Quantitative morphometry on paraffin-embedded tissue sections
  • Processing, embedding, sectioning, and staining of tissue sections for Laser Capture Microdissection available in both paraffin-embedded and frozen tissues
  • IHC or special staining on FFPE (formalin-fixed, paraffin-embedded) tissues or tissues fixed in other fixatives
  • IHC or special staining on cell preparations 
  • Frozen tissue preparation using cryostat or sliding microtome, including embedding, sectioning and staining (routine staining, special staining and immunohistochemical staining)
  • Methods-development services to adapt and evaluate the suitability of commercially available antibodies to rodent tissues

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Imaging Services
  • Photography, light and fluorescent microscopy, microphotography/digital and film, Leica DMRA2 Photomicroscope with fluorescence
  • Photomicrography and gross photography are available in support of NCTR investigators for both publication and presentation purposes
  • Gross photography system
  • Image analysis including microdensitometry, planar morphometry, particle analysis, cell proliferation and apoptotic indices using Positive Pixel Count Algorithm and Nuclear Algorithm (Aperio)
  • Digital macrophotography (digitized images are stored on a server located at the NCTR)

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Clinical Chemistry, Hematology and Urinalysis Tests
  • 5'-Nucleotidas
  • Activated Clotting Time (ACT)
  • Alanine Aminotransferase     
  • Albumin
  • Alkaline Phosphatase
  • Amylase
  • Aspartate Aminotransferase
  • Bile acids, total
  • Bilirubin, total
  • Blood Urea Nitrogen
  • Bone Marrow Count
  • Calcium
  • Chloride
  • Cholesterol, Total and HDL
  • Complete Blood Count
  • Creatine Kinase 
  • Creatinine
  • Differential Leukocyte Count
  • Erythrocyte Morphology 
  • Gamma-Glutamyl Transferase
  • Glucose
  • Glutamate-Dehydrogenase
  • Heinz Body Stain
  • Hematocrit
  • Hemoglobin
  • Howell-Jolly Bodies
  • Lactate
  • Lactate Dehydrogenase
  • Lipase
  • MCH
  • MCHC
  • MCV
  • Microalbumin
  • Micronuclei w/Acridine Orange 
  • Microscopic Urinalysis
  • NAG-N-Aceyl-B-D-Glucosaminidase
  • Non-Esterified Fatty Acids
  • Phospholipids C
  • Phosphorus
  • Platelets
  • Potassium
  • Red Cell Count
  • Reticulocyte Count
  • Sodium
  • Sorbitol Dehydrogenase
  • Total protein: Biuret
  • Triglycerides
  • Urinalysis Qualitative Tests
  • Urine Calcium
  • Urine Chloride
  • Urine Phosphorous
  • Urine Potassium
  • Urine Protein
  • Urine Sodium
  • White Cell Count
  • White Cell Differential
  • Wright-Giemsa Stain

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  • Alpha_Fetoprotein
  • Corticosterone
  • Cortisol
  • CrossLaps-ELISA
  • DHEA
  • DHEA-SO4
  • Estradiol
  • IL-2
  • IL-4
  • IL-5
  • IL-6IL-10
  • IL-12
  • IFN Gamma
  • TNF-alpha
  • IGFBP-3
  • Inhibin B
  • Insulin
  • Leptin
  • Ostocalcin
  • Rat IL 1 Alpha
  • Rat/Mouse Ghrelin (Total)
  • Rat Prolacin
  • Reverse T3 RIA
  • T3 Triiodothyronine
  • T4 Thyroxin
  • Total Testosterone
  • TSH

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Coagulation Testing
  • Protime
  • aPTT
  • Thrombin time
  • Herparin
  • Factor Assays
  • Fibrinogen
  • Protein C
  • Protein S

With versatile instrumentation many species specific esoteric tests can be adapted to run on Clinical Pathology's instruments. Please contact Clinical Pathology for tests not listed.

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Contact FDA

National Center for Toxicological Research

Food and Drug Administration

3900 NCTR Road

Jefferson, AR 72079

Page Last Updated: 07/15/2014
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