Docket Management
Docket: 03N-0059 - Pharmaceutical Current Good Manufacturing Practices for the 21st Century: A Risk-Based Approach
Comment Number: EC -22

Accepted - Volume 1

Comment Record
Commentor Miss Ann Mallon Date/Time 2003-04-06 14:03:53
Organization Pem
Category Health Professional

Comments for FDA General
Questions
1. General Comments Dear Mark McClellan M.D Ph.D., I am deeply concerned to hear that you are using sensitive species for five animal tests (skin corrosion, irritation experiments, skin absorption, phototoxicity and pyrogenicity) It is unethical and cruel to put restraining devices around rabbits and put corrosive chemicals on their shaven skin. The chemical is considered to be corrosive if it eats through the skin, burning away several layers of tissue. Like all the other animal tests, no painkillers or aesthetics are provided. Humane and relevant non-animal tests do exist. Human skin tests, such as EpiDerm (Trademark) and EpiSkin (Trademark) which use artificial skin, can measure chemical corrosivity. The method called Corrositex (TM)uses a protein membrane which is designed to function like skin. Regarding skin irritation tests, Canada accepts skin-patch tests as it is non-invasive and relates directly to people. Before reaching this stage, the chemical would have successfully passed non-animal methods of testing (computer models and other test-tube studies) to make sure that it does not have any harmful properties. I feel it is deeply wrong to smear chemicals on the backs of shaved rats. An internationally accepted non-animal test for skin absorption measure the passage of of a test chemical into and across skin to a fluid reservoir using real or artificial skin. Phototoxicity experiments causes swelling and sores from test chemicals being applied to patches of shaved skin. The 3T3 NRU Phototoxicity, on the other hand, is a non-animl test which exposes cells to a test chemical in the presence and absence of light. The degree to which the light absorbes a special neutral red dye tells us the toxicity of a test chemical. Breathing problems, circulatory and orghan failure and fatal shock often result from the pyrogenicity test on animals (injection of a test substance into the bloodstream of rabits). A non-animal test-tube method, the in vitro pyrogen test, provides accurate and cruelty-free information as to whether drugs are contaminated with fever and inflammation causing substances (pyrogens). I would be grateful if you could switch to these the cruelty-free non-animal tests today. Thank you very much indeed for taking the time to read this letter an I look forward to hearing from you shortly. Yours sincerely, Ann Mallon




EC -22