FISH Technology and its Application to Gene Amplification Analysis

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Table of Contents

FISH Technology and its Application to Gene Amplification Analysis

Genetic Testing

FISH Fluorescence In Situ Hybridization

FISH: Indirect FISH

FISH: Direct FISH

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FISH Applications

FISH Probes

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FISH - Rearrangement Detection

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Gene Amplification in Cancer

Gene Amplification

Genetic Tests for Gene Amplification

Limitations of Methods for Genetic Amplification Detection

Benefits and Limitations of Southern Blot Based Testing

Benefits and Limitations of IHC

Benefits and Limitations of FISH Based Testing

Tissue Section Based Analysis and Analytical Validation

Her2/neu Amplification in Breast Cancer

Scoring of Amplification Events Varies with Methods

Her2/neu Not Amplified 2 her2 signals vs. 2 chromosome 17s = 1.0

Her2/neu Not Amplified 2 her2 signals vs. 2 chromosome 17s = 1.0

Her2/neu Not Amplified Equal but variable number of her2 signals vs. chromosome 17 signals = 1.0

Her2/neu Amplified 4 her2 signals vs. 3 chromosome 17s = 1.33

Her2/neu Amplified 4 her2 signals vs. 2 chromosome 17s = 2.0

Her2/neu Not Amplified 5-6 her2 signals vs. 2 chromosome 17s = 3.0

Her2/neu Amplified 6-10 her2 signals vs. 2 chromosome 17s = 5.0

Her2/neu Amplified in Thin Prep from Fine Needle Aspirate 5 her2 signals vs. 2 chromosome 17s = 2.5

FISH Quality Assurance and PT

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Author: Michael Watson