FDA Logo Link U.S. Food and Drug AdministrationNational Center for Toxicological Research
U.S. Department of Health and Human Services
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Ashraf A. Khan, Ph.D., Microbiologist, Division of Microbiology, FDA, National Center for Toxicological Research

Aligarh University, B.S., 1979, Chemistry, Aligarh, India
Aligarh University, M.S., 1982, Biochemistry, Aligarh, India
Central Drug Research Institute, M. Phil., 1983, Microbiology/Biochemistry, Lucknow, India
Central Drug Research Institute, Ph.D., 1987, Microbiology/Biochemistry, Lucknow, India

Experience:

  • 1985-1988, Assistant Professor, Rajendra Agricultural University, Pusa, India
  • 1988-1992, Post-doctoral Research Fellow, Department of Biological Sciences, Oakland University, Rochester, Michigan.
  • 1991-1992, Lecturer, Department of Biological Sciences, Oakland University, Rochester, Michigan.
  • 1992-present, Microbiologist, Division of Microbiology, National Center for Toxicological Research, FDA, Jefferson, Arkansas.

Honors:

  • Group Recognition Award, Food and Drug Administration, 1998.
  • FDA Scientific Achievement Award, 2000.

Current Primary Research Interests:

  • Molecular biology of food-borne pathogens
  • Isolation and characterization of antibiotic resistant genes from food-borne pathogens
  • Molecular mechanisms of drug resistance genes
  • Development of molecular methods for the detection of food-borne pathogens and anaerobic microorganisms
  • Polymerase Chain Reaction
  • Molecular cloning of metabolic and toxin genes and their biochemical characterization
  • Protein purification and expression

Most Recent Publications (from a total of 40 publications):

  • Khan, A.A., Nawaz, M.S., Khan, S.A., and Cerniglia, C.E. "Identification of Aeromonas trota (hybridization group 13) by amplification of the aerolysin gene using polymerase chain reaction." Mol. Cell. Probes, 13:93-98 (1999).
  • Khan, S.A., Nawaz, M.S., Khan, A.A., and Cerniglia, C.E. "Direct in-gel hybridization of digoxigenin-labeled non-radioactive probes." Mol. Cell. Probes, 13:233-237 (1999).
  • Khan, S.A., Nawaz, M.S., Khan, A.A., and Cerniglia, C.E. "Simultaneous detection of erythromycin-resistant methylase genes ermA and ermC from Staphylococcus spp. by multiplex PCR." Mol. Cell Probes, 13: 381-387 (1999).
  • Nawaz, M.S., Khan, A.A., Khan, S.A., Paine, D.D., Pothuluri, J.V., and Cerniglia, C.E. "Biochemical and molecular characterization of erythromycin-resistant avian Staphylococcus spp. isolated from chickens." Poultry Sci., 78: 1191-1197 (1999).
  • Khan, A.A., Nawaz, M.S., Khan, S.A., and Cerniglia, C.E. "Detection of multidrug-resistant Salmonella typhimurium DT104: A virulent pathogen by multiplex polymerase chain reaction." FEMS Microbiol. Lett., 182: 355-360 (2000).
  • Khan, S.A., Nawaz, M.S., Khan, A.A., and Cerniglia, C.E. "Transfer of erythromycin resistance from poultry to human clinical strains of Staphylococcus aureus." J. Clin. Microbiol., 38: 1832-1838 (2000).
  • Khan, S.A., Nawaz, M.S., Khan, A.A., Steele, R.S. and Cerniglia, C.E. "Characterization of erythromycin resistant methylase genes from multiple drug resistant Staphylococcus spp. isolated from the milk of lactating cattle. Am. J. Vet. Res. (2000) (In press).
  • Wang, R.-F., Cao, W.-W., Khan, A.A., and Cerniglia, C.E. "Cloning, sequencing, and expression of the gene encoding enolase from Cunninghamella elegans." Mycol. Res., 104: 175-179 (2000).
  • Wang, R.-F., Cao, W.-W., Khan, A.A., and Cerniglia, C.E. "Cloning, sequencing, and expression in Escherichia coli of a cytochrome P450 gene from Cunninghamella elegans." FEMS Microbiol. Lett. (2000) (In press).

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