Row Drug Metabolites Fish species Dep-time t1/2-hr est Sample Ave Weight (gm) Ave Water temp (°C) Water Dosage Route Comments Protein binding Cl Vc Vd Authors Date Citation Title Genus Species Drug Class t1/2-detail Wt gm-detail Water temp (°C)-detail Dosage-detail Method Authors-red Row
1 Paraoxon   Rainbow trout       blood, plasma 120 12 Freshwater 75 ng/ml  5 d BATH t1/2 > 24 hrs in cannulated fish.  Within first 25 hrs about 1/4 dose is eliminated.  Peak at less than 4 hr (113 ug).  Residues peaked by 24 hrs with a plateau at approx 100 ng/ml remaining constant during the rest of the 140 hr exposure.   28.1 L/kg/h     Abbas R, Hayton WL 1997 Toxicology and Applied Pharmacology 145:192-201 A physiologically based pharmacokinetic and pharmacodynamic model for paraoxon in rainbow trout. Oncorhynchus mykiss Organic-Phosphorus-Pesticide >25 h 100-140 g 12 C 75 ng/ml for 5 d Absorbance Abbas R, Hayton WL 1
2 Paraoxon   Rainbow trout       brain 120 12 Freshwater 75 ng/ml  5 d BATH  Residues peaked by 24 hrs with a plateau at approx 400 ng/m remaining constant during the rest of the 140 hr exposure.         Abbas R, Hayton WL 1997 Toxicology and Applied Pharmacology 145:192-201 A physiologically based pharmacokinetic and pharmacodynamic model for paraoxon in rainbow trout. Oncorhynchus mykiss Organic-Phosphorus-Pesticide >25 h 100-140 g 12 C 75 ng/ml for 5 d Absorbance Abbas R, Hayton WL 2
3 Paraoxon   Rainbow trout       heart 120 12 Freshwater 75 ng/ml  5 d BATH  Residues peaked by 24 hrs with a plateau at approx 400 ng/m remaining constant during the rest of the 140 hr exposure.         Abbas R, Hayton WL 1997 Toxicology and Applied Pharmacology 145:192-201 A physiologically based pharmacokinetic and pharmacodynamic model for paraoxon in rainbow trout. Oncorhynchus mykiss Organic-Phosphorus-Pesticide >25 h 100-140 g 12 C 75 ng/ml for 5 d Absorbance Abbas R, Hayton WL 3
4 Paraoxon   Rainbow trout       kidney 120 12 Freshwater 75 ng/ml  5 d BATH  Residues peaked by 24 hrs with a plateau at approx 80 ng/m remaining constant during the rest of the 140 hr exposure.         Abbas R, Hayton WL 1997 Toxicology and Applied Pharmacology 145:192-201 A physiologically based pharmacokinetic and pharmacodynamic model for paraoxon in rainbow trout. Oncorhynchus mykiss Organic-Phosphorus-Pesticide >25 h 100-140 g 12 C 75 ng/ml for 5 d Absorbance Abbas R, Hayton WL 4
5 Paraoxon   Rainbow trout       liver 120 12 Freshwater 75 ng/ml  5 d BATH  Residues peaked by 24 hrs with a plateau at approx 900 ng/m remaining constant during the rest of the 140 hr exposure.         Abbas R, Hayton WL 1997 Toxicology and Applied Pharmacology 145:192-201 A physiologically based pharmacokinetic and pharmacodynamic model for paraoxon in rainbow trout. Oncorhynchus mykiss Organic-Phosphorus-Pesticide >25 h 100-140 g 12 C 75 ng/ml for 5 d Absorbance Abbas R, Hayton WL 5
6 Paraoxon   Rainbow trout   1   blood, plasma 345 12 Freshwater 75 ng/ml  up to 120 hrs BATH Concentrations ng/ml during exposure- 0.25h=70.3, 0.5h=85.6, 1h=88.7, 1.5h=92.4, 2h=101.7, 4h=104.3, 6h=103.8, 12h=102.6, 24h=101.1, 48h=99.2, 72h=98.9 (rapid uptake and then plateau).   2.78 L/kg/h   1.04 L/kg Abbas R, Schultz IR, Doddapaneni S, Hayton WL 1996 Toxicology and Applied Pharmacology 136:194-199 Toxicokinetics of parathion and paraoxon in rainbow trout after intravascular administration and water exposure. Oncorhynchus mykiss Organic-Phosphorus-Pesticide 0.535 h 140-550 g 12 C 75 ng/ml  up to 120 hrs GC Abbas R, Schultz IR, Doddapaneni S, Hayton WL 6
7 Paraoxon   Rainbow trout   1   blood, plasma 440 12 Freshwater 5.7  mg/kg sd IVC Catheterized fish. Vd for paraoxon is 1080 mg/kg 48% 3.02 L/kg/h   1.080 L/kg Abbas R, Schultz IR, Doddapaneni S, Hayton WL 1996 Toxicology and Applied Pharmacology 136:194-199 Toxicokinetics of parathion and paraoxon in rainbow trout after intravascular administration and water exposure. Oncorhynchus mykiss Organic-Phosphorus-Pesticide 0.528 h 1330-550 g 12 C 5.7  mg/kg sd GC Abbas R, Schultz IR, Doddapaneni S, Hayton WL 7
8 Parathion Paraoxon Rainbow trout   56   blood, plasma 440 12 Freshwater 10 mg/kg sd IVC Paraoxon is a metabolite of parathion; Vd for parathion = 1370 ml/kg.  Catheterized fish. 98% 0.021 L/kg/h   1.370 L/kg Abbas R, Schultz IR, Doddapaneni S, Hayton WL 1996 Toxicology and Applied Pharmacology 136:194-199 Toxicokinetics of parathion and paraoxon in rainbow trout after intravascular administration and water exposure. Oncorhynchus mykiss Organic-Phosphorus-Pesticide 56.1 h 1330-550 g 12 C 10 mg/kg sd GC Abbas R, Schultz IR, Doddapaneni S, Hayton WL 8
9 Parathion Paraoxon Rainbow trout   57   blood, plasma 345 12 Freshwater 75 ng/ml  up to 120 hrs BATH Paraoxon is a metabolite of parathion;  Parathion concentration reached approx1.0 ug/ml in first 24 hr then declined to 0.4 slowly over the next 100 hr.   0.183 L/kg/h   1.280 L/kg Abbas R, Schultz IR, Doddapaneni S, Hayton WL 1996 Toxicology and Applied Pharmacology 136:194-199 Toxicokinetics of parathion and paraoxon in rainbow trout after intravascular administration and water exposure. Oncorhynchus mykiss Organic-Phosphorus-Pesticide 57.3 h 140-550 g 12 C 75 ng/ml  up to 120 hrs GC Abbas R, Schultz IR, Doddapaneni S, Hayton WL 9
10 Oxytetracycline   Chinook salmon   88   blood 350 11 Saltwater 50 mg/kg sd IVC Lower Vd than expected in both spp, maybe because implanted cannula; bioavailability of OTC after PO are 24.84% (salmon) and 30.30% (trout) which are higher than normal; Vd= 0.89 l/kg  - Chinook in seawater.  OTC conc in blood went from 1000 ug/ml  to approx 8 ug/ml in first 200 h, down to 1 by 900 h. AUC= 7126.8 ug h/ml       0.89 L/kg Abedini S, Namdari R, Law FCP 1998 Aquaculture 162:23-32 Comparative pharmacokinetics and bioavailability of oxytetracycline in rainbow trout and Chinook salmon. Oncorhynchus tshawytscha Antibiotic-Tetracycline 88.29 h 350 g 11 C 50 mg/kg sd HPLC Abedini S, Namdari R, Law FCP 10
11 Oxytetracycline   Chinook salmon   428   blood 350 11 Saltwater 50 mg/kg sd PO Chinook in seawater. Conc in bloold went from about 8 ug/ml to 2.5 in first 200 h and down to 1 by 900 h.         Abedini S, Namdari R, Law FCP 1998 Aquaculture 162:23-32 Comparative pharmacokinetics and bioavailability of oxytetracycline in rainbow trout and Chinook salmon. Oncorhynchus tshawytscha Antibiotic-Tetracycline 428.19 h 350 g 11 C 50 mg/kg sd HPLC Abedini S, Namdari R, Law FCP 11
12 Oxytetracycline   Rainbow trout   94   blood 350 11 Freshwater 50 mg/kg sd IVC Lower Vd than expected in both spp, maybe because implanted cannula; bioavailability of OTC after PO are 24.84% (salmon) and 30.30% (trout) which are higher than normal;  Vd= 0.87l/kg.  Conc went from 1000 ug/ml  to approx 6 in first 200 h, then to 1 by 900h. AUC = 7781.2 ug h/ml.       0.87 L/kg Abedini S, Namdari R, Law FCP 1998 Aquaculture 162:23-32 Comparative pharmacokinetics and bioavailability of oxytetracycline in rainbow trout and Chinook salmon. Oncorhynchus mykiss Antibiotic-Tetracycline 94.22 h 350 g 11 C 50 mg/kg sd HPLC Abedini S, Namdari R, Law FCP 12
13 Oxytetracycline   Rainbow trout   479   blood 350 11 Freshwater 50 mg/kg sd PO Conc went from approx 8 ug/ml to 4 in first 200 h then to 1 by 900 h.         Abedini S, Namdari R, Law FCP 1998 Aquaculture 162:23-32 Comparative pharmacokinetics and bioavailability of oxytetracycline in rainbow trout and Chinook salmon. Oncorhynchus mykiss Antibiotic-Tetracycline 479.43 h 350 g 11 C 50 mg/kg sd HPLC Abedini S, Namdari R, Law FCP 13
14 Sulfachlorpyridazine   Channel catfish   4 est blood, plasma 312 22 Freshwater 60 mg/kg sd PO t 1/2 est from graph.  C-max 8.7 by 237 min.         Alavi FK, Rolf LL, Clarke CR 1993 J. Veterinary Pharmacology and Therapeutics 16:232-236 The pharmacokinetics of sulfachlorpyridazine in channel catfish, Ictalurus punctatus. Ictalurus punctatus Antibiotic-Sulfa 230  min (est from graph) 312+/-220g 22+/-2C 60 mg/kg sd Absorbance-Bratton&Marshall Alavi FK, Rolf LL, Clarke CR 14
15 Sulfachlorpyridazine   Channel catfish   5   blood, plasma 312 22 Freshwater 60 mg/kg sd IC conc went from approx 1100 ug/ml to 80 in first 200 min and down to 20 by 1h. 4.63-14.20% 0.077 L/kg/h   0.043 L/kg Alavi FK, Rolf LL, Clarke CR 1993 J. Veterinary Pharmacology and Therapeutics 16:232-236 The pharmacokinetics of sulfachlorpyridazine in channel catfish, Ictalurus punctatus. Ictalurus punctatus Antibiotic-Sulfa 230+/-65 min 312+/-220g 22+/-2C 60 mg/kg sd Absorbance-Bratton&Marshall Alavi FK, Rolf LL, Clarke CR 15
16 Malachite green Leukomalachite green Rainbow trout       gill 70 18 Freshwater 1 mg/l 60 min BATH Residues decreased fairly rapidly during first 320 days reaching 260 by d41, and below 1 ng/g after 7th month. T 1/2 in the 20 d range initially, with later slow decline in the 50d range.         Alborali L, Sangiorgi E, Leali M, Guadagnini PF, Sicura S 1997 Rivista Italiana di Acquacoltura 32:45-60 The persistence of malachite green in the edible tissue of rainbow trout (Oncorhynchus mykiss).  Oncorhynchus mykiss Organic-Dye   60-80 g 18 C 1 mg L 1 h HPLC Alborali L, Sangiorgi E, Leali M, Guadagnini PF, Sicura S 16
17 Malachite green Leukomalachite green Rainbow trout       kidney 70 18 Freshwater 1 mg/l 60 min BATH Max residue on d 30=1650 ng/g, declined to below 1 ng/g after 41 d.         Alborali L, Sangiorgi E, Leali M, Guadagnini PF, Sicura S 1997 Rivista Italiana di Acquacoltura 32:45-60 The persistence of malachite green in the edible tissue of rainbow trout (Oncorhynchus mykiss).  Oncorhynchus mykiss Organic-Dye   60-80 g 18 C 1 mg L 1 h HPLC Alborali L, Sangiorgi E, Leali M, Guadagnini PF, Sicura S 17
18 Malachite green Leukomalachite green Rainbow trout       muscle 70 18 Freshwater 1 mg/l 60 min BATH Residues high for 34 days - above 1,000 ng/g.  Decreased to 200 ng at the 4th month, around 100 by 150d, slowly declining to below 10 ng/g by 9th month. T 1/2 in the 40-50 d range.         Alborali L, Sangiorgi E, Leali M, Guadagnini PF, Sicura S 1997 Rivista Italiana di Acquacoltura 32:45-60 The persistence of malachite green in the edible tissue of rainbow trout (Oncorhynchus mykiss).  Oncorhynchus mykiss Organic-Dye   60-80 g 18 C 1 mg L 1 h HPLC Alborali L, Sangiorgi E, Leali M, Guadagnini PF, Sicura S 18
19 Malachite green Leukomalachite green Rainbow trout       skin 70 18 Freshwater 1 mg/l 60 min BATH Residues decreased fairly rapidly during first 20 days, to about 2500 ng/g, d50=approx 1000, 200 ng/g round the 4th month, below 1 ng/g at day 283.  t 1/2 in the 50d range.         Alborali L, Sangiorgi E, Leali M, Guadagnini PF, Sicura S 1997 Rivista Italiana di Acquacoltura 32:45-60 The persistence of malachite green in the edible tissue of rainbow trout (Oncorhynchus mykiss).  Oncorhynchus mykiss Organic-Dye   60-80 g 18 C 1 mg L 1 h HPLC Alborali L, Sangiorgi E, Leali M, Guadagnini PF, Sicura S 19
20 Malachite green   Rainbow trout   15   blood, serum 199 8 Freshwater 1.6 mg/L 40 min BATH t 1/2 alpha =  2.15 h.  C max 13 mg/ml 0h.         Alderman DJ, Clifton-Hadley RS 1993 J. Fish Diseases 16:297-311 Malachite green: a pharmacokinetic study in rainbow trout, Oncorhynchus mykiss (Walbaum). Oncorhynchus mykiss Organic-Dye 15 h 199 +/- 20 g 7.7 +/-0.4 C 1.6 mg/L 40 min Absorbance Alderman DJ, Clifton-Hadley RS 20
21 Malachite green   Rainbow trout   348   blood, serum 241 16 Freshwater 1.6 mg/L 40 min BATH t 1/2 alpha =  7.33 h  C max 13.5 mg/ml 0h.         Alderman DJ, Clifton-Hadley RS 1993 J. Fish Diseases 16:297-311 Malachite green: a pharmacokinetic study in rainbow trout, Oncorhynchus mykiss (Walbaum). Oncorhynchus mykiss Organic-Dye 348 h 241 +/- 33g 15.8 +/-0.8 C 1.6 mg/L 40 min Absorbance Alderman DJ, Clifton-Hadley RS 21
22 Malachite green   Rainbow trout   139   kidney 241 16 Freshwater 1.6 mg/L 40 min BATH t 1/2 alpha =  6.46 h. C max348 ppm 0h.         Alderman DJ, Clifton-Hadley RS 1993 J. Fish Diseases 16:297-311 Malachite green: a pharmacokinetic study in rainbow trout, Oncorhynchus mykiss (Walbaum). Oncorhynchus mykiss Organic-Dye 139 h 241 +/- 33g 15.8 +/-0.8 C 1.6 mg/L 40 min Absorbance Alderman DJ, Clifton-Hadley RS 22
23 Malachite green   Rainbow trout   720   kidney 199 8 Freshwater 1.6 mg/L 40 min BATH t 1/2 alpha = 11.78 h. C mx 8 ppm 0h.         Alderman DJ, Clifton-Hadley RS 1993 J. Fish Diseases 16:297-311 Malachite green: a pharmacokinetic study in rainbow trout, Oncorhynchus mykiss (Walbaum). Oncorhynchus mykiss Organic-Dye 720 h 199 +/- 20 g 7.7 +/-0.4 C 1.6 mg/L 40 min Absorbance Alderman DJ, Clifton-Hadley RS 23
24 Malachite green   Rainbow trout   136   liver 241 16 Freshwater 1.6 mg/L 40 min BATH t 1/2 alpha =  1.98 h.  C max 16.5 ppm 0h.         Alderman DJ, Clifton-Hadley RS 1993 J. Fish Diseases 16:297-311 Malachite green: a pharmacokinetic study in rainbow trout, Oncorhynchus mykiss (Walbaum). Oncorhynchus mykiss Organic-Dye 136 h 241 +/- 33g 15.8 +/-0.8 C 1.6 mg/L 40 min Absorbance Alderman DJ, Clifton-Hadley RS 24
25 Malachite green   Rainbow trout   331   liver 199 8 Freshwater 1.6 mg/L 40 min BATH t 1/2 alpha =  2.10 h C max 9 ppm 0h.         Alderman DJ, Clifton-Hadley RS 1993 J. Fish Diseases 16:297-311 Malachite green: a pharmacokinetic study in rainbow trout, Oncorhynchus mykiss (Walbaum). Oncorhynchus mykiss Organic-Dye 331 h 199 +/- 20 g 7.7 +/-0.4 C 1.6 mg/L 40 min Absorbance Alderman DJ, Clifton-Hadley RS 25
26 Malachite green   Rainbow trout   70   muscle 241 16 Freshwater 1.6 mg/L 40 min BATH t 1/2 alpha =  8.35 h.  Cmax 10.8 ppm  at 90-120 min         Alderman DJ, Clifton-Hadley RS 1993 J. Fish Diseases 16:297-311 Malachite green: a pharmacokinetic study in rainbow trout, Oncorhynchus mykiss (Walbaum). Oncorhynchus mykiss Organic-Dye 69.36 h 241 +/- 33g 15.8 +/-0.8 C 1.6 mg/L 40 min Absorbance Alderman DJ, Clifton-Hadley RS 26
27 Malachite green   Rainbow trout   93   muscle 199 8 Freshwater 1.6 mg/L 40 min BATH t 1/2 alpha =  5.43 h  Cmax 7.8 ppm at 90-120 min.         Alderman DJ, Clifton-Hadley RS 1993 J. Fish Diseases 16:297-311 Malachite green: a pharmacokinetic study in rainbow trout, Oncorhynchus mykiss (Walbaum). Oncorhynchus mykiss Organic-Dye 92.64 h 199 +/- 20 g 7.7 +/-0.4 C 1.6 mg/L 40 min Absorbance Alderman DJ, Clifton-Hadley RS 27
28 Malachite green   Atlantic salmon       eggs     Not specified 1 mg/l  1 h * BATH Residues in eggs taken from treated adults ranged d1=4.16-3.15, d2-d7= 0.72-3.6, d24-d41 = 0.21-2.29 ug/g.         Allen JL 1990 Investigations in Fish Control U.S. Dept. of the Interior, Fish and Wildlife Service, Bureau of Sport Fisheries and Wildlife. 101:1-4 Residues of Malachite Green in muscle, eggs, and fry of treated Atlantic salmon and Chinook salmon. Salmo salar Organic-Dye   Not specifed Not specified 1 ppm for 1 h for 20-47 treatments Absorbance Allen JL 28
29 Malachite green   Atlantic salmon       muscle     Not specified 1 mg/l  1 h * BATH Residues highest (about 1.0 to 2.5 ug/g) in fish sampled 1 or 2 d after last treatment. At 5-7 d after treatment residues =0.64-2.22 ug/g.  Residues after 24-41 d = 0.91-0.33 ug/g.         Allen JL 1990 Investigations in Fish Control U.S. Dept. of the Interior, Fish and Wildlife Service, Bureau of Sport Fisheries and Wildlife. 101:1-4 Residues of Malachite Green in muscle, eggs, and fry of treated Atlantic salmon and Chinook salmon. Salmo salar Organic-Dye   Not specifed Not specified 1 ppm for 1 h for 20-47 treatments Absorbance Allen JL 29
30 Malachite green   Chinook salmon       eggs     Not specified 1 mg/l  1 h * BATH Residues in eggs taken from treated adults.  Mean residues post treatment d1= 0.8-1.8, d6=0.97-1.86, d12-d18= 1.16-1.69 ug/g after 27-30 treatments.         Allen JL 1990 Investigations in Fish Control U.S. Dept. of the Interior, Fish and Wildlife Service, Bureau of Sport Fisheries and Wildlife. 101:1-4 Residues of Malachite Green in muscle, eggs, and fry of treated Atlantic salmon and Chinook salmon. Oncorhynchus tshawytscha Organic-Dye   Not specifed Not specified 1 ppm for 1 h for 27-30 treatments Absorbance Allen JL 30
31 Malachite green   Chinook salmon       muscle     Not specified 1 mg/l  1 h * BATH Mean residues post treatment d1= 1.4, d6=0.61, d12-d18= 0.66 ug/g after 27-30 treatments.         Allen JL 1990 Investigations in Fish Control U.S. Dept. of the Interior, Fish and Wildlife Service, Bureau of Sport Fisheries and Wildlife. 101:1-4 Residues of Malachite Green in muscle, eggs, and fry of treated Atlantic salmon and Chinook salmon. Oncorhynchus tshawytscha Organic-Dye   Not specifed Not specified 1 ppm for 1 h for 27-30 treatments Absorbance Allen JL 31
32 Malachite green   Chinook salmon       whole body     Not specified 1 mg/l  1 h * BATH Residues in newly hatched fry d1=0.9-1.04, d6=1.0-1.16, 12d-18d = 1.01-1.03 ug/g after 27-30 treatments.         Allen JL 1990 Investigations in Fish Control U.S. Dept. of the Interior, Fish and Wildlife Service, Bureau of Sport Fisheries and Wildlife. 101:1-4 Residues of Malachite Green in muscle, eggs, and fry of treated Atlantic salmon and Chinook salmon. Oncorhynchus tshawytscha Organic-Dye   Not specifed Not specified 1 ppm for 1 h for 27-30 treatments Absorbance Allen JL 32
33 Malachite green Leuco MG Rainbow trout       muscle+skin   12 Freshwater 1 mg/L 1h sd BATH {14C]Malachite Green. Mean total residue d0= 1 ug/g, 5days withdrawal=0.4 ug/g. mean Leuko residue d0=47 ug/g, d5 withdrawal = 39 ug/g. the leuko metabolite was about 70-80% of the total residue.         Allen JL, Gofus JE, Meinertz JR 1994 J. AOAC International 77:553-557 Determination of malachite green residues in the eggs, fry, and adult muscle tissue of rainbow trout (Oncorhynchus mykis). Oncorhynchus mykiss Organic-Dye   adult 12 1 mg/L 1h sd Rad Allen JL, Gofus JE, Meinertz JR 33
34 Malachite green   Rainbow trout   48 est muscle   14 Freshwater 0.1 mg/l  24 hr sd BATH Residues at 0 d = 1.87, 1 d = 1.17, 2 d = 0.72, 5 d = 0.28, 6 d = 0.22 ug/g after exposure for 24 hr.         Allen JL, Hunn JB 1986 Veterinary and Human Toxicology 28 (supp 1):21-24 Fate and distribution studies of some drugs used in aquaculture. Oncorhynchus mykiss Organic-Dye 48 h est Not Specified 14 0.1 mg/l for 24 hr sd Absorbance Allen JL, Hunn JB 34
35 MS-222 meta-aminobenzoate ester Channel catfish       blood     Freshwater 100 mg/l BATH Tricaine  methane sulphonate.  After 0.5, 1.0, and 1.5 hour Withdrawal times in freshwater, residues of ms-222 were present in blood and brain.  Liver and kidney samples could not  be analyzed because the clean-up method used in this study was ineffective for these tissues         Allen JL, Luhning CW, Harmen PD (1970) 1970 Investigations in Fish Control. U.S. Dept. of the Interior, Fish and Wildlife Service, Bureau of Sport Fisheries and Wildlife. SH157.7.I58 no.42 Identification  of MS-222 residues in selected fish tissues by thin layer chromatography. Ictalurus punctatus Anesthetic-Tricaine   Not Specified Not Specified 100ppm Absorbance-Bratton&Marshall, TLC Allen JL, Luhning CW, Harmen PD (1970) 35
36 MS-222 meta-aminobenzoate ester Channel catfish       brain     Freshwater 100 mg/l BATH Tricaine  methane sulphonate.   After 0.5, 1.0, and 1.5 hour Withdrawal times in freshwater, residues of ms-222 were present in blood and brain.  Liver and kidney samples could not  be analyzed because the clean-up method used in this study was ineffective for these tissues         Allen JL, Luhning CW, Harmen PD (1970) 1970 Investigations in Fish Control. U.S. Dept. of the Interior, Fish and Wildlife Service, Bureau of Sport Fisheries and Wildlife. SH157.7.I58 no.42 Identification  of MS-222 residues in selected fish tissues by thin layer chromatography. Ictalurus punctatus Anesthetic-Tricaine   Not Specified Not Specified 100ppm Absorbance-Bratton&Marshall, TLC Allen JL, Luhning CW, Harmen PD (1970) 36
37 MS-222 meta-aminobenzoate ester Channel catfish       muscle     Freshwater 100 mg/l BATH Tricaine methane sulphonate.  In muscle spiked with 0.2-10.0 ppm of ms-222 recoveries ranged from 25-60%         Allen JL, Luhning CW, Harmen PD (1970) 1970 Investigations in Fish Control. U.S. Dept. of the Interior, Fish and Wildlife Service, Bureau of Sport Fisheries and Wildlife. SH157.7.I58 no.42 Identification  of MS-222 residues in selected fish tissues by thin layer chromatography. Ictalurus punctatus Anesthetic-Tricaine   Not Specified Not Specified 100ppm Absorbance-Bratton&Marshall, TLC Allen JL, Luhning CW, Harmen PD (1970) 37
38 MS-222 Acetylated metabolite Muskellung nearly 24 h 24 est muscle 1286 10 Freshwater 150 mg/l  till deeply anesthetized BATH Tricaine methane sulphonate.  Compared HPLC and TLC methods.  Total MS222 free and acetylated residues ( ppm) at 0 h = 10.7, 2 h =  2.2, 24 h =2.0.  (control had 1.2).         Allen JL, Luhning CW, Harmen PD (1972) 1972 Investigations in Fish Control. U.S. Dept. of the Interior, Fish and Wildlife Service, Bureau of Sport Fisheries and Wildlife. SH157.7.I58 no.45 Residues of MS-222 in Northern Pike, Muskellunge, and Walleye. Esox masquinongy Anesthetic-Tricaine 24 544-770 gm 10 C 150 ppm  till deeply anesthetized Absorbance-Bratton&Marshall, TLC Allen JL, Luhning CW, Harmen PD (1972) 38
39 MS-222 Acetylated metabolite Northern pike nearly 24 h 2 est muscle 1286 17 Freshwater 150 mg/l  till deeply anesthetized BATH Tricaine methane sulphonate.  Compared HPLC and TLC methods.  Total MS222 free and acetylated residues ( ppm) at 0 h =8.1 , 2 h =  0.5, 6 h =  0.5, 24 h =1.0.  (control had 2.1).         Allen JL, Luhning CW, Harmen PD (1972) 1972 Investigations in Fish Control. U.S. Dept. of the Interior, Fish and Wildlife Service, Bureau of Sport Fisheries and Wildlife. SH157.7.I58 no.45 Residues of MS-222 in Northern Pike, Muskellunge, and Walleye. Esox lucius Anesthetic-Tricaine 2 690-1180 gm 17 C 150 ppm  till deeply anesthetized Absorbance-Bratton&Marshall, TLC Allen JL, Luhning CW, Harmen PD (1972) 39
40 MS-222 Acetylated metabolite Northern pike nearly 24 h 6 est muscle 1286 12 Freshwater 150 mg/l  till deeply anesthetized BATH Tricaine methane sulphonate.  Compared HPLC and TLC methods.  Total MS222 free and acetylated residues ( ppm) at 0 h =22.0 , 6 h =  0.7, 24 h =0.7.  (control had 1.4)..         Allen JL, Luhning CW, Harmen PD (1972) 1972 Investigations in Fish Control. U.S. Dept. of the Interior, Fish and Wildlife Service, Bureau of Sport Fisheries and Wildlife. SH157.7.I58 no.45 Residues of MS-222 in Northern Pike, Muskellunge, and Walleye. Esox lucius Anesthetic-Tricaine 6 690-1180 gm 12 C 150 ppm  till deeply anesthetized Absorbance-Bratton&Marshall, TLC Allen JL, Luhning CW, Harmen PD (1972) 40
41 MS-222 Acetylated metabolite Northern pike nearly 24 h 6 est muscle 1286 17 Freshwater 150 mg/l  till deeply anesthetized BATH Tricaine methane sulphonate.  Compared HPLC and TLC methods.  Total MS222 free and acetylated residues ( ppm) at 0 h =12.2 , 6 h =  2.0, 24 h =1.1.  (control had 1.8).         Allen JL, Luhning CW, Harmen PD (1972) 1972 Investigations in Fish Control. U.S. Dept. of the Interior, Fish and Wildlife Service, Bureau of Sport Fisheries and Wildlife. SH157.7.I58 no.45 Residues of MS-222 in Northern Pike, Muskellunge, and Walleye. Esox lucius Anesthetic-Tricaine 6 690-1180 gm 17 C 150 ppm  till deeply anesthetized Absorbance-Bratton&Marshall, TLC Allen JL, Luhning CW, Harmen PD (1972) 41
42 MS-222 Acetylated metabolite Northern pike nearly 24 h 24 est muscle 1286 8 Freshwater 150 mg/l  till deeply anesthetized BATH Tricaine methane sulphonate.  Compared HPLC and TLC methods.  Total MS222 free and acetylated residues ( ppm) at 0 h =11.3 , 2 h =  6.2, 24 h =1.3  (control had 2.0).         Allen JL, Luhning CW, Harmen PD (1972) 1972 Investigations in Fish Control. U.S. Dept. of the Interior, Fish and Wildlife Service, Bureau of Sport Fisheries and Wildlife. SH157.7.I58 no.45 Residues of MS-222 in Northern Pike, Muskellunge, and Walleye. Esox lucius Anesthetic-Tricaine 24 690-1180 gm 8 C 150 ppm  till deeply anesthetized Absorbance-Bratton&Marshall, TLC Allen JL, Luhning CW, Harmen PD (1972) 42
43 MS-222 Acetylated metabolite Northern pike nearly 24 h 24 est muscle 1286 12 Freshwater 150 mg/l  till deeply anesthetized BATH Tricaine methane sulphonate.  Compared HPLC and TLC methods.  Total MS222 free and acetylated residues ( ppm) at 0 h =9.1 , 2 h =  5.7, 24 h =2. (Control had 2.6).         Allen JL, Luhning CW, Harmen PD (1972) 1972 Investigations in Fish Control. U.S. Dept. of the Interior, Fish and Wildlife Service, Bureau of Sport Fisheries and Wildlife. SH157.7.I58 no.45 Residues of MS-222 in Northern Pike, Muskellunge, and Walleye. Esox lucius Anesthetic-Tricaine 24 690-1180 gm 12 C 150 ppm  till deeply anesthetized Absorbance-Bratton&Marshall, TLC Allen JL, Luhning CW, Harmen PD (1972) 43
44 MS-222 Acetylated metabolite Walleye