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U.S. Department of Health and Human Services

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Volume IV - 5.5 Quantification

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Orientation and Training

Food and Drug Administration

DOCUMENT NO.:

IV-05
VERSION NO.: 1.3

Section 5 - Pesticides Analysis

EFFECTIVE DATE: 10/01/2003REVISED: 02/14/2013

A. Objective

  1. Using the PAM section 504, introduce general concepts for measuring gas chromatographic peaks used in routine quantification of pesticide analytes.
  2. Using the PAM section 504, introduce the strategy for quantification of complex mixtures or multicomponent compounds. 

B.  Assignment

  1. Under a trainer’s guidance, make standard and sample injections of various analytes, including mixtures, perform the appropriate calculations and discuss the results completely.

C.  References                    

  1. U.S. Food & Drug Administration, Center for Food Safety and Applied Nutrition, Office of Plant, Dairy Foods and Beverages. Makovi, C. M. (Ed.), McMahon, B. M. (Ed. Emerita). (1994). Pesticide analytical manual-multiresidue methods (3rd ed., Vol. I, section 504- Quantitation, (updated 10/99) R.O.W. Sciences, Inc.

D. Questions

  1. What are the two types of manual quantitation discussed in the PAM Section 504?
  2. When is peak height the measurement technique of choice?
  3. What can be a major pitfall with using electronic integration techniques?
  4. Explain the purpose of “peak width” and “peak threshold” settings in electronic integration.
  5. According to PAM Section 504, explain briefly a strategy that gives good quantitation of PCB.