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Food
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BAM Media M34: Casamino Acids-Yeast Extract-Salts (CA-YE) Broth
January 2001
Bacteriological Analytical Manual
M34
Casamino Acids-Yeast Extract-Salts Broth (CA-YE)
(Gorbach)
| Casamino acids | 20 g |
| Yeast extract | 6 g |
| NaCl | 2.5 g |
| K2HPO4 | 8.71 g |
| Trace salts solution (below), optional |
1 ml |
| Distilled water | 1 liter |
Adjust pH so that value after autoclaving is 8.5 ± 0.2. Autoclave 15 min at 121°C.
Trace salts solution (optional)
| MgSO4 | 50 g |
| MnCl2 | 5 g |
| FeCl2 | 5 g |
| Distilled water | 1 liter |
Suspend ingredients. Add enough 0.1 N H2SO4 to dissolve. Sterilize by filtration through 0.45 µm membrane. Add 1 ml to 1 liter base. Dispense 10 ml portions into 50 ml flasks.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.
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