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Food
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BAM Media M36: Cell Growth Medium
January 2001
Bacteriological Analytical Manual
M36
Cell Growth Medium
Mix the following sterile solutions aseptically:
| Minimal essential medium with Hanks' salts (MEMH) |
1 liter |
| L-15 medium (Leibovitz) (containing 100 units penicillin G, 100 5g streptomycin, and 50 5g gentamicin per ml) |
1 liter |
Mix on magnetic stirrer, filter through 0.20 µm membrane, and dispense into sterile 2 liter Erlenmeyer flask. Final pH, 7.5. Cap and store at 5°C.
Just before use add:
| Fetal bovine serum | 200 ml |
| NaHCO3 (7.5%) | 50 ml |
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.
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