BAM Media M189: Cellobiose-Colistin (CC) Agar

May 2004

Bacteriological Analytical Manual

M189
Cellobiose-Colistin (CC) Agar

Solution 1

Adjust to pH 7.6. Boil to dissolve agar. Cool to 48-55°C.

*1000X Dye stock solution

For consistent medium color, use dye solution rather than repeatedly weighing out dry dyes. Dissolve dyes in ethanol for 4% (w/v) stock solution. Using 1 ml of this solution per liter of mCPC agar gives 40 mg bromthymol blue and 40 mg cresol red per liter.

Solution 2

Dissolve cellobiose in distilled water by heating gently. Cool. Add antibiotics and filter sterilize.

Add Solution 2 to cooled Solution 1, mix, and dispense into petri dishes. Final color, dark green to green-brown.

NOTE: This medium, like TCBS, is very inhibitory and does not require autoclaving. Medium may be stored 2 weeks at refrigeration temperatures.