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Food
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BAM Media M37: Cetrimide Agar
January 2001
Bacteriological Analytical Manual
M37
Cetrimide Agar
| Pancreatic digest of gelatin | 20.0 g |
| Magnesium chloride | 1.4 g |
| Potassium sulfate | 10.0 g |
| Agar | 13.6 g |
| Cetrimide (cetyl trimethyl ammonium bromide) |
0.3 g |
Suspend 45.3 g of ingredients or commercial powder (PseudoselTM, BBL; Becton-Dickinson) in 1 liter of purified water. Add 10 ml glycerol. Mix well. Heat to boiling, agitating frequently; maintain boiling for 1 min to dissolve powder.
Autoclave at 118°C for 15 min.
Final pH, 7.2 ± 0.2. Bacto cetrimide agar base plus glycerol (Difco) is a similar medium.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.
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