-
Food
-
BAM Media M40: Christensen's Urea Agar
January 2001
Bacteriological Analytical Manual
M40
Christensen's Urea Agar
Base
| Peptone | 1 g |
| NaCl | 5 g |
| Dextrose | 1 g |
| KH2PO4 | 2 g |
| Phenol red (6 ml of 1:500 solution) |
0.012 g |
| Agar | 15 g |
| Distilled water | 900 ml |
Dissolve all ingredients except urea in 900 ml water (basal medium).
For halophilic Vibrio spp., add extra 15 g NaCl (final NaCl concentration, 2%).
Autoclave 15 min at 121°C. Cool to 50-55°C.
Urea concentrate
| Urea | 20 g |
| Distilled water | 100 ml |
Dissolve urea in 100 ml water.
Filter-sterilize; add aseptically to cooled basal medium. Mix. Final pH, 6.8 ± 0.1. Dispense to sterile tubes or petri dishes. Slant tubes for 2 cm butt and 3 cm slant.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.
-
-