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Food
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BAM Media M87: Lysine Decarboxylase Broth
January 2001
Bacteriological Analytical Manual
M87
Lysine Decarboxylase Broth
(Falkow)
(for Salmonella)
| Gelysate or peptone | 5 g |
| Yeast extract | 3 g |
| Glucose | 1 g |
| L-Lysine | 5 g |
| Bromcresol purple | 0.02 g |
| Distilled water | 1 liter |
Heat until dissolved. Dispense 5 ml portions into 16 x 125 mm screw-cap tubes. Autoclave loosely capped tubes 15 min at 121°C. Screw the caps on tightly for storage and after inoculation. Final pH, 6.8 ± 0.2.
For halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.
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