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BAM Media M76: Lauryl Tryptose (LST) Broth

January 2001

Bacteriological Analytical Manual

M76
Lauryl Tryptose (LST) Broth

Tryptose or trypticase	20 g
Lactose	5 g
K₂HPO₄	2.75 g
KH₂PO₄	2.75 g
NaCl	5 g
Sodium lauryl sulfate	0.1 g
Distilled water	1 liter

Dispense 10 ml portions into 20 x 150 mm tubes containing inverted 10 x 75 mm fermentation tubes. Autoclave 15 min at 121°C. Final pH, 6.8 ± 0.2.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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